The purpose of the Tissue Processing and Cell Culture Core (Core C) component of this PPG is to provide centralized expert handling of clinical samples obtained from human subjects by the Clinical Core B. The tissue processing and cell culture activities of Core C will convert the clinical samples into materials (airway cells, fluid, slides, etc.) required for the research proposed in all three Projects. This core will also utilize explanted lungs to culture and provide human primary smooth muscle and epithelial cells to the projects in this PPG. There are many advantages to having these activities done by a single Core facility rather than in each investigator's own laboratory. Foremost, the personnel staffing of the Core are highly experienced in performing the required procedures in a meticulous and standardized manner. In addition Core C will be responsible for ensuring that samples are properly characterized, inventoried, stored, and that data is mantained in a data base with appropriate access to PPG investigators and other cores. In this capacity, the Core will allow for each clinical sample to be utilized to its full potential in multiple Projects. This Core will interact closely with all Cores in this PPG (Core A, B and D) to maintain the extensive database required to catalog and manage the processed clinical materials and generate coherent links among experimental results obtained with these materials.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL081064-04
Application #
7802835
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2009-04-01
Budget End
2010-03-31
Support Year
4
Fiscal Year
2009
Total Cost
$191,291
Indirect Cost
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195
Allawzi, Ayed M; Vang, Alexander; Clements, Richard T et al. (2018) Activation of Anoctamin-1 Limits Pulmonary Endothelial Cell Proliferation via p38-Mitogen-activated Protein Kinase-Dependent Apoptosis. Am J Respir Cell Mol Biol 58:658-667
Reichard, Andrew; Asosingh, Kewal (2018) The role of mitochondria in angiogenesis. Mol Biol Rep :
Ghosh, Arnab; Garee, Greer; Sweeny, Elizabeth A et al. (2018) Hsp90 chaperones hemoglobin maturation in erythroid and nonerythroid cells. Proc Natl Acad Sci U S A 115:E1117-E1126
Comhair, Suzy A A; Bochenek, Grazyna; Baicker-McKee, Sara et al. (2018) The utility of biomarkers in diagnosis of aspirin exacerbated respiratory disease. Respir Res 19:210
Johnson, Collin G; Stober, Vandy P; Cyphert-Daly, Jaime M et al. (2018) High molecular weight hyaluronan ameliorates allergic inflammation and airway hyperresponsiveness in the mouse. Am J Physiol Lung Cell Mol Physiol :
Majors, Alana K; Chakravarti, Ritu; Ruple, Lisa M et al. (2018) Nitric oxide alters hyaluronan deposition by airway smooth muscle cells. PLoS One 13:e0200074
Sweeny, Elizabeth A; Singh, Anuradha Bharara; Chakravarti, Ritu et al. (2018) Glyceraldehyde-3-phosphate dehydrogenase is a chaperone that allocates labile heme in cells. J Biol Chem 293:14557-14568
Reichard, Andrew; Wanner, Nicholas; Stuehr, Eric et al. (2018) Quantification of airway fibrosis in asthma by flow cytometry. Cytometry A 93:952-958
Asosingh, Kewal; Weiss, Kelly; Queisser, Kimberly et al. (2018) Endothelial cells in the innate response to allergens and initiation of atopic asthma. J Clin Invest 128:3116-3128
Dai, Yue; Haque, Mohammad Mahfuzul; Stuehr, Dennis J (2017) Restricting the conformational freedom of the neuronal nitric-oxide synthase flavoprotein domain reveals impact on electron transfer and catalysis. J Biol Chem 292:6753-6764

Showing the most recent 10 out of 123 publications