? Core B This core focuses on qualitative and quantitative analyses of genes, proteins, peptides and small drug compounds using conventional biochemical methods coupled with highly sensitive mass spectrometry (MS) and Laser Capture Microdissection (LCM) for sample collection. It will serve as a central resource for LCM and qRT-PCR (UNTHSC) and MS analysis of covalent protein modifications (UTHSCSA) of CNS samples from the 3 projects. The core will receive raw samples from investigators, most often these will be in the form of punches from specific brain regions of interest. LCM will be used to harvest defined populations of cells from brain sections allowing the projects to conduct experiments using samples that are more specific than punched samples. Retrograde track tracing, juxtacellular labeling, immunohistochemistry, and the expression of reporter molecules will be used to identify neurons of interest.
? Core B The Analytical Core (Core B) will provide technical and scientific support in the areas of genomics and proteomics for all three Projects to achieve efficiency of cost, effort and equipment usage. Core B will provide technical support for sample collection and preparation allowing all projects to use the Laser Capture Microdissection (LCM) Core at the UNT Health Science Center at Fort Worth and the Mass Spectrometry (MS) Core at the UT Health Science Center at San Antonio.
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