The Analytical Core will provide a variety of specialized analytical services for the investigators ofthe Program Project to assist in the conduct of the molecular, cellular and in vivo studies. TheAnalytical Core will be a collaborative effort between units at the Salk Institute under the direction ofDr. Ronald Evans, and one located at the University of California, San Diego under Dr. JosephWitztum. The overall goal of the Analytical Core is to take advantage of specialized resources andexpertise to provide investigators with selected core services that will assist in the research missionof each Project.
The Aims of this Core are:
Specific Aim 1 : To provide high throughput quantitative PCR analysis (HT-QPCR) of selectedtarget genes, such as nuclear receptors or target genes that impact inflammation andatherosclerosis. This platform will allow rapid exploratory mechanism of impact of variousperturbations on nuclear hormone receptor expression in macrophages and B-1 cells, as well as inother cells or tissue.
Specific Aim 2 : To provide high throughput and highly sensitive analysis of cytokines andchemokines in small sample volumes, such as plasma or culture fluids, through use of Bioplexsuspension array proteomic profiling.
Specific Aim 3 : To provide immunological services including the generation of antigen-specificantisera and monoclonal antibodies, preparation of reagents and performance of a variety ofimmunological assays, as for example, antibody levels in plasma of murine models under study.
Specific Aim 4 : To provide quantitative and qualitative analysis of lipids and lipoproteins, to prepareisolated lipoprotein fractions needed, such as LDL, as well as provision of standardized modifiedlipoproteins, such as oxidized LDL and mmLDL for use by all investigators.
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