Abstract

The Histology &Imaging Core will provide a central site and umbrella authority, which together with satellite infrastructure on this campus confers necessary services, instrumentation, supervision and training for PPG members and personnel within their laboratories. The Core's two major functions are (i) to assist characterization of undifferentiated and differentiated phenotypes of mouse and human embryonic stem cells and their derivatives (all Subprojects), and (2) to assist with the unambiguous histologic characterization of engrafted and regenerated myocardium following stem cell transplantation (Subprojects 1, 3, 4). To achieve these goals the core will assume a training and advisory role while providing services including fixation, embedding and sectioning;routine, histochemical and immunohistochemical staining;widefield and fluorescence microscopy;laser-scanning confocal and multi-photon microscopy;electron microscopy; microangiography. By maintaining an organized online database, processing of images to reconstruct and qualitatively analyze the extent of stem cell engraftment in transplanted myocardium will be provided.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL089471-02
Application #
8121379
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2010-08-01
Budget End
2011-07-31
Support Year
2
Fiscal Year
2010
Total Cost
$139,492
Indirect Cost

  Institution

Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226

  Publications

Colunga, Thomas; Dalton, Stephen (2018) Building Blood Vessels with Vascular Progenitor Cells. Trends Mol Med 24:630-641
Garitaonandia, Ibon; Amir, Hadar; Boscolo, Francesca Sesillo et al. (2015) Increased risk of genetic and epigenetic instability in human embryonic stem cells associated with specific culture conditions. PLoS One 10:e0118307
Kolander, Kurt D; Holtz, Mary L; Cossette, Stephanie M et al. (2014) Epicardial GATA factors regulate early coronary vascular plexus formation. Dev Biol 386:204-15
Lakshmikanthan, Sribalaji; Zieba, Bartosz J; Ge, Zhi-Dong et al. (2014) Rap1b in smooth muscle and endothelium is required for maintenance of vascular tone and normal blood pressure. Arterioscler Thromb Vasc Biol 34:1486-94
Mallanna, Sunil K; Duncan, Stephen A (2013) Differentiation of hepatocytes from pluripotent stem cells. Curr Protoc Stem Cell Biol 26:Unit 1G.4.
Gundry, Rebekah L; Riordon, Daniel R; Tarasova, Yelena et al. (2012) A cell surfaceome map for immunophenotyping and sorting pluripotent stem cells. Mol Cell Proteomics 11:303-16
Sepac, Ana; Si-Tayeb, Karim; Sedlic, Filip et al. (2012) Comparison of cardiomyogenic potential among human ESC and iPSC lines. Cell Transplant 21:2523-30
Van Orman, Jordan R; Si-Tayeb, Karim; Duncan, Stephen A et al. (2012) Induction of cardiomyogenesis in human embryonic stem cells by human embryonic stem cell-derived definitive endoderm. Stem Cells Dev 21:987-94
Fisher, Joseph B; Kim, Min-Su; Blinka, Steven et al. (2012) Stress-induced cell-cycle activation in Tip60 haploinsufficient adult cardiomyocytes. PLoS One 7:e31569
Cayo, Max A; Cai, Jun; DeLaForest, Ann et al. (2012) JD induced pluripotent stem cell-derived hepatocytes faithfully recapitulate the pathophysiology of familial hypercholesterolemia. Hepatology 56:2163-71

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