Core B: Outcomes Core (Core B: Minami) The Outcomes Core has 4 objectives 1) To provide surgical models of myocardial infarction to serve as a basis for studying the effects of stem-cell therapy in myocardial infarct repair. 2) To provide in-vivo physiology endpoint measurements using echocardiography, MRI, and microspheres to determine functional improvement post-engraftment 3) To provide basic tissue preparation and sectioning to allow each project to study endpoints at the fissue level. 4) To develop a canine model of myocardial infarction and grafting techniques to allow us to study the effects of embryonic-stem cell derived cardiomyocytes and mesenchymal stem cells in large animal models so that we can be one step closer to bringing this therapy to the clinical setting.

Public Health Relevance

The long term goal of this Core is to assist investigators in this project to study how stem cells and embryonic stem cell-derived cardiomyocytes improve cardiac function so that eventually, these cells can be potentially used as treatment for patients with ischemic cardiomyopathy.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL094374-02
Application #
8327803
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
2
Fiscal Year
2011
Total Cost
$366,298
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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Hansen, Katrina J; Favreau, John T; Gershlak, Joshua R et al. (2017) Optical Method to Quantify Mechanical Contraction and Calcium Transients of Human Pluripotent Stem Cell-Derived Cardiomyocytes. Tissue Eng Part C Methods 23:445-454
Palpant, Nathan J; Wang, Yuliang; Hadland, Brandon et al. (2017) Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis. Cell Rep 20:1597-1608
Palpant, Nathan J; Pabon, Lil; Friedman, Clayton E et al. (2017) Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells. Nat Protoc 12:15-31
Yang, Xiulan; Murry, Charles E (2017) One Stride Forward: Maturation and Scalable Production of Engineered Human Myocardium. Circulation 135:1848-1850

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