Abstract

Core B: Outcomes Core (Core B: Minami) The Outcomes Core has 4 objectives 1) To provide surgical models of myocardial infarction to serve as a basis for studying the effects of stem-cell therapy in myocardial infarct repair. 2) To provide in-vivo physiology endpoint measurements using echocardiography, MRI, and microspheres to determine functional improvement post-engraftment 3) To provide basic tissue preparation and sectioning to allow each project to study endpoints at the fissue level. 4) To develop a canine model of myocardial infarction and grafting techniques to allow us to study the effects of embryonic-stem cell derived cardiomyocytes and mesenchymal stem cells in large animal models so that we can be one step closer to bringing this therapy to the clinical setting.

Public Health Relevance

The long term goal of this Core is to assist investigators in this project to study how stem cells and embryonic stem cell-derived cardiomyocytes improve cardiac function so that eventually, these cells can be potentially used as treatment for patients with ischemic cardiomyopathy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL094374-04
Application #
8485645
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
4
Fiscal Year
2013
Total Cost
$342,931
Indirect Cost
$137,158

  Institution

Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Liu, Yen-Wen; Chen, Billy; Yang, Xiulan et al. (2018) Human embryonic stem cell-derived cardiomyocytes restore function in infarcted hearts of non-human primates. Nat Biotechnol 36:597-605
Hofsteen, Peter; Robitaille, Aaron Mark; Strash, Nicholas et al. (2018) ALPK2 Promotes Cardiogenesis in Zebrafish and Human Pluripotent Stem Cells. iScience 2:88-100
Neidig, Lauren E; Weinberger, Florian; Palpant, Nathan J et al. (2018) Evidence for Minimal Cardiogenic Potential of Stem Cell Antigen 1-Positive Cells in the Adult Mouse Heart. Circulation 138:2960-2962
Leonard, Andrea; Bertero, Alessandro; Powers, Joseph D et al. (2018) Afterload promotes maturation of human induced pluripotent stem cell derived cardiomyocytes in engineered heart tissues. J Mol Cell Cardiol 118:147-158
Hansen, Katrina J; Laflamme, Michael A; Gaudette, Glenn R (2018) Development of a Contractile Cardiac Fiber From Pluripotent Stem Cell Derived Cardiomyocytes. Front Cardiovasc Med 5:52
Eschenhagen, Thomas; Bolli, Roberto; Braun, Thomas et al. (2017) Cardiomyocyte Regeneration: A Consensus Statement. Circulation 136:680-686
Hansen, Katrina J; Favreau, John T; Gershlak, Joshua R et al. (2017) Optical Method to Quantify Mechanical Contraction and Calcium Transients of Human Pluripotent Stem Cell-Derived Cardiomyocytes. Tissue Eng Part C Methods 23:445-454
Palpant, Nathan J; Wang, Yuliang; Hadland, Brandon et al. (2017) Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis. Cell Rep 20:1597-1608
Palpant, Nathan J; Pabon, Lil; Friedman, Clayton E et al. (2017) Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells. Nat Protoc 12:15-31
Yang, Xiulan; Murry, Charles E (2017) One Stride Forward: Maturation and Scalable Production of Engineered Human Myocardium. Circulation 135:1848-1850

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