Abstract

The purpose of Core C is to provide state-of-the-art measurements of reactive oxygen species (ROS), reduced glutathione (GSH), glutathione disulfide (GSSG), oxidative modifications of cysteine residues of redox-sensitive proteins in cells, tissues and membranes for participants of the PPG. Core C will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy, High Perfonnance Liquid Chromatography (HPLC) and nano-LC MS/MS analysis. This Core facility has existed since 1999 and has supported numerous studies of superoxide (O2*), hydrogen peroxide (H2O2) and NO in vascular cells and tissue, and it was an essential part of the previous program project studies by Drs. Harrison, Griendling, Taylor and Jo (1-10). This facility will significantly enhance the work of the individual laboratories. and will enhance collaborations between participants of the PPG

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL095070-03
Application #
8308443
Study Section
Special Emphasis Panel (ZHL1)
Project Start
Project End
Budget Start
2011-08-01
Budget End
2012-07-31
Support Year
3
Fiscal Year
2011
Total Cost
$171,989
Indirect Cost

  Institution

Name
Emory University
Department
Type
DUNS #
066469933
City
Atlanta
State
GA
Country
United States
Zip Code
30322

  Publications

Okwan-Duodu, Derick; Hansen, Laura; Joseph, Giji et al. (2018) Impaired Collateral Vessel Formation in Sickle Cell Disease. Arterioscler Thromb Vasc Biol 38:1125-1133
Hu, Shuhong; Liu, Yifei; You, Tao et al. (2018) Vascular Semaphorin 7A Upregulation by Disturbed Flow Promotes Atherosclerosis Through Endothelial ?1 Integrin. Arterioscler Thromb Vasc Biol 38:335-343
Heath, Jack M; Fernandez Esmerats, Joan; Khambouneheuang, Lucky et al. (2018) Mechanosensitive microRNA-181b Regulates Aortic Valve Endothelial Matrix Degradation by Targeting TIMP3. Cardiovasc Eng Technol 9:141-150
Forrester, Steven J; Kikuchi, Daniel S; Hernandes, Marina S et al. (2018) Reactive Oxygen Species in Metabolic and Inflammatory Signaling. Circ Res 122:877-902
Paredes, Felipe; Sheldon, Kely; Lassègue, Bernard et al. (2018) Poldip2 is an oxygen-sensitive protein that controls PDH and ?KGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer. Proc Natl Acad Sci U S A 115:1789-1794
Xu, Qian; Kulkarni, Amol A; Sajith, Ayyiliath M et al. (2018) Design, synthesis, and biological evaluation of inhibitors of the NADPH oxidase, Nox4. Bioorg Med Chem 26:989-998
Paredes, Felipe; Suster, Izabela; Martin, Alejandra San (2018) Poldip2 takes a central role in metabolic reprograming. Oncoscience 5:130-131
Lee, Grace Sanghee; Salazar, Hector F; Joseph, Giji et al. (2018) Osteopontin isoforms differentially promote arteriogenesis in response to ischemia via macrophage accumulation and survival. Lab Invest :
Simmons, Craig A; Jo, Hanjoong (2018) Editorial: Special Issue on Heart Valve Mechanobiology : New Insights into Mechanical Regulation of Valve Disease and Regeneration. Cardiovasc Eng Technol 9:121-125
Williams, Holly C; Ma, Jing; Weiss, Daiana et al. (2018) The cofilin phosphatase slingshot homolog 1 restrains angiotensin II-induced vascular hypertrophy and fibrosis in vivo. Lab Invest :

Showing the most recent 10 out of 125 publications