The innate immune system plays critical roles in maintaining a healthy lung and in shaping the adaptive immune response to challenge. As for most biological processes, the extent, pattern, and duration of inflammation are controlled by a balance between positive and negative factors. Our preliminary data indicates that stromelysin-2 (MMP-10), a member of the matrix metalloproteinase gene family, functions to govern the extent of inflammation in the lung by controlling the influx and activation state of infiltrated macrophages. In response to toxic injury or bacterial infection, stromelysin-2 expression is induced, and when production peaked in wildtype mice, MmpIO'^'mice died or were moribund. The difference in mortality between wildtype and null mice was not associated with a difference in bacterial clearance. Rather, MmpW'' had more severe inflammation in lung tissue than did wildtype mice. Furthermore, data from cell culture models indicate that most stromelysin-2 expression is produced by infiltrated macrophages, and compared to cells from infected wildtype mice, macrophages from MmpIO'''mice expressed reduced levels of some immunosuppressive factors, such as IL-10. Gene expression arrays studies in other models (smoke exposure) suggest that stromelysin-2 is needed for activation of several immune pathways. Based on these data, we hypothesize that stromelysln-2 functions to moderate lung inflammation and immune processes by controlling the activation state of infiltrated macrophages and, in turn, downstream Immune processes. To study the role of stromelysin-2 in more detail, we propose to 1) identify the subpopulation of macrophages and the activation status affected by stromelysin-2;2) determine the relative roles of epithelial- and macrophage-derived stromelysin-2;and 3) assess the role of stromelysin-2 in governing the macrophage response to bacterial infection. This project complements the overall theme of this Program to explore mechanisms linking innate immunity in the lung to downstream adaptive responses, and several interactions are proposed with the other three projects and both scientific cores.
These studies will characterize a novel, fundamental mechanism controlling macrophage activation and the resolution of inflammation. Knowledge from this work may provide an effective strategy to limit inflammationassociated damage not only in lung, but in all tissues.
| Rohani, Maryam G; Dimitrova, Elizabeth; Beppu, Andrew et al. (2018) Macrophage MMP10 Regulates TLR7-Mediated Tolerance. Front Immunol 9:2817 |
| Maisel, Katharina; Merrilees, Mervyn J; Atochina-Vasserman, Elena N et al. (2018) Immune Checkpoint Ligand PD-L1 Is Upregulated in Pulmonary Lymphangioleiomyomatosis. Am J Respir Cell Mol Biol 59:723-732 |
| Han, H; Roan, F; Johnston, L K et al. (2018) IL-33 promotes gastrointestinal allergy in a TSLP-independent manner. Mucosal Immunol 11:394-403 |
| Evanko, Stephen P; Chan, Christina K; Johnson, Pamela Y et al. (2018) The biochemistry and immunohistochemistry of versican. Methods Cell Biol 143:261-279 |
| Han, Hongwei; Roan, Florence; Ziegler, Steven F (2017) The atopic march: current insights into skin barrier dysfunction and epithelial cell-derived cytokines. Immunol Rev 278:116-130 |
| Kang, Inkyung; Harten, Ingrid A; Chang, Mary Y et al. (2017) Versican Deficiency Significantly Reduces Lung Inflammatory Response Induced by Polyinosine-Polycytidylic Acid Stimulation. J Biol Chem 292:51-63 |
| Sheih, A; Parks, W C; Ziegler, S F (2017) GM-CSF produced by the airway epithelium is required for sensitization to cockroach allergen. Mucosal Immunol 10:705-715 |
| Kashiwagi, Mariko; Hosoi, Junichi; Lai, Jen-Feng et al. (2017) Direct control of regulatory T cells by keratinocytes. Nat Immunol 18:334-343 |
| Secor, Patrick R; Michaels, Lia A; Smigiel, Kate S et al. (2017) Filamentous Bacteriophage Produced by Pseudomonas aeruginosa Alters the Inflammatory Response and Promotes Noninvasive Infection In Vivo. Infect Immun 85: |
| Han, Hongwei; Ziegler, Steven F (2017) Intradermal administration of IL-33 induces allergic airway inflammation. Sci Rep 7:1706 |
Showing the most recent 10 out of 83 publications
