Abstract

Core B will have three specific aims.
Specific Aim 1 is to harvest, characterize and propagate human lung microvascular endothelial cells from subjects undergoing lobectomy or pneumonectomy at MCG Health Inc.;provide said cells to investigators in all four projects for permeability, analytical and other molecular studies.
Specific Aim 2 is to create and supply to all four projects mice with ALI induced by intra-tracheal instillation of gram positive or gram negative bacterial products.
Specific Aim 3 is to perform in vivo lung capillary permeability (Evan's Blue Dye Method) measurements and lung function (airway mechanics) measurements for studies designed by each of the four projects.

Public Health Relevance

The goal of this Core is to help the four studies develop new approaches to the management of acute lung injury and acute respiratory distress syndrome, conditions that carry a high degree of mortality. It will provide the tools needed to investigate means of restoring the known dysfunction and increased permeability of lung capillary vessels.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL101902-03
Application #
8508294
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
3
Fiscal Year
2013
Total Cost
$371,279
Indirect Cost
$123,760

  Institution

Name
Georgia Regents University
Department
Type
DUNS #
966668691
City
Augusta
State
GA
Country
United States
Zip Code
30912

  Publications

Kovacs-Kasa, Anita; Kim, Kyung Mi; Cherian-Shaw, Mary et al. (2018) Extracellular adenosine-induced Rac1 activation in pulmonary endothelium: Molecular mechanisms and barrier-protective role. J Cell Physiol 233:5736-5746
Gross, Christine M; Kellner, Manuela; Wang, Ting et al. (2018) LPS-induced Acute Lung Injury Involves NF-?B-mediated Downregulation of SOX18. Am J Respir Cell Mol Biol 58:614-624
Chepurnova, D A; Samoilova, E V; Anisimov, A A et al. (2018) Compounds of IL-6 Receptor Complex during Acute Lung Injury. Bull Exp Biol Med 164:609-611
Yang, Guang; Pillich, Helena; White, Richard et al. (2018) Listeriolysin O Causes ENaC Dysfunction in Human Airway Epithelial Cells. Toxins (Basel) 10:
Barman, Scott A; Chen, Feng; Li, Xueyi et al. (2018) Galectin-3 Promotes Vascular Remodeling and Contributes to Pulmonary Hypertension. Am J Respir Crit Care Med 197:1488-1492
Barabutis, Nektarios; Dimitropoulou, Christiana; Gregory, Betsy et al. (2018) Wild-type p53 enhances endothelial barrier function by mediating RAC1 signalling and RhoA inhibition. J Cell Mol Med 22:1792-1804
Wang, Ting; Gross, Christine; Desai, Ankit A et al. (2017) Endothelial cell signaling and ventilator-induced lung injury: molecular mechanisms, genomic analyses, and therapeutic targets. Am J Physiol Lung Cell Mol Physiol 312:L452-L476
Barabutis, Nektarios; Khangoora, Vikramjit; Marik, Paul E et al. (2017) Hydrocortisone and Ascorbic Acid Synergistically Prevent and Repair Lipopolysaccharide-Induced Pulmonary Endothelial Barrier Dysfunction. Chest 152:954-962
Dou, Huijuan; Feher, Attila; Davila, Alec C et al. (2017) Role of Adipose Tissue Endothelial ADAM17 in Age-Related Coronary Microvascular Dysfunction. Arterioscler Thromb Vasc Biol 37:1180-1193
Song, Shanshan; Ayon, Ramon J; Yamamura, Aya et al. (2017) Capsaicin-induced Ca2+ signaling is enhanced via upregulated TRPV1 channels in pulmonary artery smooth muscle cells from patients with idiopathic PAH. Am J Physiol Lung Cell Mol Physiol 312:L309-L325

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