Abstract

Core C is designed as the Shared Resources Core. It will provide a number of centralized services to meet the key glycoscience analytical techniques required to achieve the aims set forth in each Project of this PEG. This core is central to the goals ofthe Program, offering several highly innovative technologies created by Program scientists specifically to interrogate glycan structure and function, and ensuring proper execution of a variety of technically-demanding procedures. The Core is divided into 4 components: (1) Glycoanalytic Component;(2) Adhesion Assay Component;(3) Ex Vivo Glycan Engineering Component;and (4) In Vitro Glycosyltransferase and Glycosidase Component. This core realizes the Program's intent to sustain an experimentally integrated effort, and ensures that data derived from glycoscience-specialized activities will be uniformly reproducible among all the Projects.

Public Health Relevance

Core C is designed as the Shared Resources Core. It will provide a number of centralized services to meet the key glycoscience analytical techniques required to achieve the aims set forth in each Project of this PEG.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL107146-04
Application #
8669084
Study Section
Special Emphasis Panel (ZHL1-CSR-H)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
4
Fiscal Year
2014
Total Cost
$270,689
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Mondal, Nandini; Dykstra, Brad; Lee, Jungmin et al. (2018) Distinct human ?(1,3)-fucosyltransferases drive Lewis-X/sialyl Lewis-X assembly in human cells. J Biol Chem 293:7300-7314
Donnelly, Conor; Dykstra, Brad; Mondal, Nandini et al. (2018) Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression. Sci Rep 8:420
Carrascal, Mylène A; Silva, Mariana; Ramalho, José S et al. (2018) Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation. Mol Oncol 12:579-593
Irons, Eric E; Lau, Joseph T Y (2018) Systemic ST6Gal-1 Is a Pro-survival Factor for Murine Transitional B Cells. Front Immunol 9:2150
Videira, Paula A; Silva, Mariana; Martin, Kyle C et al. (2018) Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration. J Immunol 201:1030-1043
Carrascal, Mylène A; Talina, Catarina; Borralho, Paula et al. (2018) Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue. BMC Cancer 18:495
Buffone Jr, Alexander; Nasirikenari, Mehrab; Manhardt, Charles T et al. (2017) Leukocyte-borne ?(1,3)-fucose is a negative regulator of ?2-integrin-dependent recruitment in lung inflammation. J Leukoc Biol 101:459-470
Dougher, Christopher W L; Buffone Jr, Alexander; Nemeth, Michael J et al. (2017) The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis. J Leukoc Biol 102:507-516
Cheng, Kai; Zhou, Yusen; Neelamegham, Sriram (2017) DrawGlycan-SNFG: a robust tool to render glycans and glycopeptides with fragmentation information. Glycobiology 27:200-205
Pachón-Peña, Gisela; Donnelly, Conor; Ruiz-Cañada, Catalina et al. (2017) A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells. Stem Cells 35:1080-1092

Showing the most recent 10 out of 69 publications