Instmctions): (Core B, Lauer) The Shared Resource Core is one of the three cores required by the Cleveland Clinic Program of Excellence in Glycoscience (CC-PEG). The primary objectives of this Core are: 1) to accelerate the pace of the individual research projects by providing them with the glycoscience skills and facilities necessary for glycosaminoglycan analysis, and 2) to provide post-doctoral fellows supported by this program with hands-on instruction in glycosaminoglycan analytical techniques as part of a close partnership with the Glycoscience Skills Development Core. The Shared Resource Core will provide services and instruction in a variety of analytical techniques, involving glycosaminoglycans: (a) extraction and purification, (b) mass, size and sulfation profiling, (c) binding partners and modifications, (d) immunohistochemistry and gene expression, and (e) smooth muscle/endothellal cell culture. These services and instructions will insure timeliness, efficiency and uniformity for all the projects in achieving their aims that require extensive analyses of glycosaminoglycans/proteoglycans, thereby facilitating meeting the overall goals of the CC-PEG to determine the roles of hyaluronan matrices in vascular pathologies.

Public Health Relevance

The Shared Resource Core will contribute to public health by providing the Individual research projects with the specialized analytical tools required for glycosaminoglycan/proteoglycan analyses. These analyses will involve clinical samples from patients with vascular disease involving diabetes, pulmonary hypertension and inflammatory bowel disease.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL107147-02
Application #
8378612
Study Section
Special Emphasis Panel (ZHL1-CSR-H)
Project Start
Project End
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
2
Fiscal Year
2012
Total Cost
$288,069
Indirect Cost
$91,132
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195
Midura, Ronald J; Cali, Valbona; Lauer, Mark E et al. (2018) Quantification of hyaluronan (HA) using a simplified fluorophore-assisted carbohydrate electrophoresis (FACE) procedure. Methods Cell Biol 143:297-316
Petrey, Aaron C; de la Motte, Carol A (2018) Hyaluronan in inflammatory bowel disease: Cross-linking inflammation and coagulation. Matrix Biol :
Schnellmann, Rahel; Sack, Ragna; Hess, Daniel et al. (2018) A Selective Extracellular Matrix Proteomics Approach Identifies Fibronectin Proteolysis by A Disintegrin-like and Metalloprotease Domain with Thrombospondin Type 1 Motifs (ADAMTS16) and Its Impact on Spheroid Morphogenesis. Mol Cell Proteomics 17:1410-1425
Mead, Timothy J; Du, Yaoyao; Nelson, Courtney M et al. (2018) ADAMTS9-Regulated Pericellular Matrix Dynamics Governs Focal Adhesion-Dependent Smooth Muscle Differentiation. Cell Rep 23:485-498
Cikach, Frank S; Koch, Christopher D; Mead, Timothy J et al. (2018) Massive aggrecan and versican accumulation in thoracic aortic aneurysm and dissection. JCI Insight 3:
Sivakumar, Aravind; Mahadevan, Aparna; Lauer, Mark E et al. (2018) Midgut Laterality Is Driven by Hyaluronan on the Right. Dev Cell 46:533-551.e5
Kim, Yeojung; West, Gail A; Ray, Greeshma et al. (2018) Layilin is critical for mediating hyaluronan 35kDa-induced intestinal epithelial tight junction protein ZO-1 in vitro and in vivo. Matrix Biol 66:93-109
Sikes, Katie J; Renner, Kristen; Li, Jun et al. (2018) Knockout of hyaluronan synthase 1, but not 3, impairs formation of the retrocalcaneal bursa. J Orthop Res 36:2622-2632
Kessler, Sean P; Obery, Dana R; Nickerson, Kourtney P et al. (2018) Multifunctional Role of 35 Kilodalton Hyaluronan in Promoting Defense of the Intestinal Epithelium. J Histochem Cytochem 66:273-287
Chen, Jee-Wei E; Pedron, Sara; Shyu, Peter et al. (2018) Influence of Hyaluronic Acid Transitions in Tumor Microenvironment on Glioblastoma Malignancy and Invasive Behavior. Front Mater 5:

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