The Core C (Physiology Core) for the MCW program project grant, entitled ?Renal Mechanisms in Blood Pressure Control?, will support program project investigators with comprehensive biochemical, microscopy, electrophysiological, and phenotyping services to assess physiological and pathophysiological function of the kidney in the control of blood pressure. The need for Core C is apparent considering the current heavy use and anticipated use in the proposed protocols. Throughout the history of the PPG, Core C has provided centralized, standardized research services that have enabled program investigators to engage in cutting edge research. The overall goal of Core C is to assist program project investigators with the application of many of the basic and more-comprehensive analyses relevant to the study of kidney physiology and its relationship to the blood pressure control at the cellular, organ, and whole animal level. Multiple approaches will be offered, including biochemical analysis, chronic blood pressure measurement by telemetry or indwelling catheters, laser-Doppler flowmetry, GFR measurement, biosensors, fluorescence probes, and electrophysiology analysis in freshly isolated tubules. Core C will serve as an invaluable resource for the project investigators to define the specific mechanisms that lead to the control of blood pressure and kidney diseases. Core staff will also assist in experimental design and training of the laboratory staff, and participate in data collection and analysis.

Public Health Relevance

Physiology Core C is an essential component of this PPG and will support all three projects. The scientific projects share the common goal of providing novel insights into the mechanisms and functional pathways underlying the development of salt-sensitive hypertension and renal damage. The overall goal of Core C is to provide all PPG project investigators access to modern equipment and technical expertise needed to perform a wide variety of high quality assays and approaches necessary to conduct proposed studies.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL116264-09
Application #
10075947
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
OH, Youngsuk
Project Start
2013-09-01
Project End
2021-12-31
Budget Start
2021-01-01
Budget End
2021-12-31
Support Year
9
Fiscal Year
2021
Total Cost
Indirect Cost
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
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Cowley Jr, Allen W (2018) Chrm3 Gene and M3 Muscarinic Receptors Contribute to Salt-Sensitive Hypertension. Hypertension 72:588-591
Palygin, Oleg; Pochynyuk, Oleh; Staruschenko, Alexander (2018) Distal tubule basolateral potassium channels: cellular and molecular mechanisms of regulation. Curr Opin Nephrol Hypertens 27:373-378

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