The scientific premise of this project is based upon the significance of salt-sensitive hypertension as a health problem and the need to understand the underlying mechanisms to enable development of more effective treatments. The studies proposed pursue mTOR signaling, which has long been known to be important in the regulation of cell cycle events and in human cancers, but has remained unexplored in hypertension. It is also recognized that mTOR complexes can participate in autoimmune responses and cardiovascular diseases and studies find they are important for renal podocyte homeostasis and tubular epithelial Na+ and K+ transport. Despite evidence that many aspects of kidney physiology known to be altered in hypertension are negatively affected by stimulation of mTOR complexes, the therapeutic benefit of inhibition of these pathways has not been studied. Indeed, remarkably little is known about the contributions of mTOR to the pathophysiology of hypertension. Our preliminary studies find that inhibition of the mTOR complexes virtually abolished salt-induced hypertension in a rat model of salt-sensitive hypertension (Dahl SS rats). Given that a high salt diet stimulates H2O2 production in the kidney of SS rats, and based on our in vitro evidence, we hypothesize that in the SS rat model of salt-sensitive hypertension enhanced intrarenal production of H2O2, largely from Nox4, enhances mTOR signaling. We propose that activated mTORC2 phosphorylates and activates SGK1; thereby increasing tubular Na+ reabsorption which results in Na+ retention and hypertension (and as studied in Project 3, enhanced inflammation). Project 1 has three Specific Aims:
Aim 1 will test the hypothesis that therapeutic suppression of the mTORC2 pathway will reduce blood pressure salt-sensitivity and renal injury in SS rats.
Aim 2 will test the hypothesis that mTORC2/SGK1 contributes to enhanced tubular Na+ reabsorption and salt-sensitivity of SS rats.
Aim 3 will test the hypothesis that elevations of intrarenal H2O2 in SS rats fed a high salt diet contribute importantly to the activation of the mTOR signaling pathway. These mechanisms are not just of academic interest since understanding of these pathways could yield novel therapeutic targets.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL116264-09
Application #
10075948
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
OH, Youngsuk
Project Start
2013-09-01
Project End
2021-12-31
Budget Start
2021-01-01
Budget End
2021-12-31
Support Year
9
Fiscal Year
2021
Total Cost
Indirect Cost
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Mattson, David L (2018) Heat stress nephropathy and hyperuricemia. Am J Physiol Renal Physiol 315:F757-F758
Abais-Battad, Justine M; Lund, Hayley; Fehrenbach, Daniel J et al. (2018) Rag1-null Dahl SS rats reveal that adaptive immune mechanisms exacerbate high protein-induced hypertension and renal injury. Am J Physiol Regul Integr Comp Physiol 315:R28-R35
Bukowy, John D; Dayton, Alex; Cloutier, Dustin et al. (2018) Do computers dream of electric glomeruli? Kidney Int 94:635
Spires, Denisha; Ilatovskaya, Daria V; Levchenko, Vladislav et al. (2018) Protective role of Trpc6 knockout in the progression of diabetic kidney disease. Am J Physiol Renal Physiol 315:F1091-F1097
Bukowy, John D; Dayton, Alex; Cloutier, Dustin et al. (2018) Region-Based Convolutional Neural Nets for Localization of Glomeruli in Trichrome-Stained Whole Kidney Sections. J Am Soc Nephrol 29:2081-2088
Regal, Jean F; Laule, Connor F; McCutcheon, Luke et al. (2018) The complement system in hypertension and renal damage in the Dahl SS rat. Physiol Rep 6:e13655
Abais-Battad, Justine M; Lund, Hayley; Fehrenbach, Daniel J et al. (2018) Parental Dietary Protein Source and the Role of CMKLR1 in Determining the Severity of Dahl Salt-Sensitive Hypertension. Hypertension :HYPERTENSIONAHA11811994
Williams, Anna Marie; Liu, Yong; Regner, Kevin R et al. (2018) Artificial intelligence, physiological genomics, and precision medicine. Physiol Genomics 50:237-243
Palygin, Oleg; Miller, Bradley S; Nishijima, Yoshinori et al. (2018) Endothelin receptor A and p66Shc regulate spontaneous Ca2+ oscillations in smooth muscle cells controlling renal arterial spontaneous motion. FASEB J :fj201800776RR
Wade, Brittany; Petrova, Galina; Mattson, David L (2018) Role of immune factors in angiotensin II-induced hypertension and renal damage in Dahl salt-sensitive rats. Am J Physiol Regul Integr Comp Physiol 314:R323-R333

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