Abstract

A key goal of biomedical investigators interested in HDL is to establish the relationships between the structure and function of the lipoprotein. The HDL Quantitation Core will provide the powerful tools of mass spectrometry and quantification of HDL particle size and concentration to PPG investigators. Core personnel have documented expertise in these areas, and their capabilities will support PPG investigators with a standardized, validated set of common assays. Core C will also optimize efficiency and cost-effectiveness by providing these services in a central laboratory.

Public Health Relevance

The HDL Quantitation Core provides Program Project Grant (PPG) investigators with precise and accurate measurement of particle size and number as well as state-of-the-art mass spectrometric analyses to test structural models of HDL.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
1P01HL128203-01A1
Application #
9073918
Study Section
Special Emphasis Panel (HLBP (JH))
Project Start
2016-09-15
Project End
2021-06-30
Budget Start
2016-09-15
Budget End
2017-06-30
Support Year
1
Fiscal Year
2016
Total Cost
$557,006
Indirect Cost
$14,500

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
079917897
City
Nashville
State
TN
Country
United States
Zip Code
37232

  Publications

Lima, Diogo B; Melchior, John T; Morris, Jamie et al. (2018) Characterization of homodimer interfaces with cross-linking mass spectrometry and isotopically labeled proteins. Nat Protoc 13:431-458
Shao, Baohai; Heinecke, Jay W (2018) Quantifying HDL proteins by mass spectrometry: how many proteins are there and what are their functions? Expert Rev Proteomics 15:31-40
Cooke, Allison L; Morris, Jamie; Melchior, John T et al. (2018) A thumbwheel mechanism for APOA1 activation of LCAT activity in HDL. J Lipid Res 59:1244-1255
He, Yi; Kothari, Vishal; Bornfeldt, Karin E (2018) High-Density Lipoprotein Function in Cardiovascular Disease and Diabetes Mellitus. Arterioscler Thromb Vasc Biol 38:e10-e16
Pourmousa, Mohsen; Song, Hyun D; He, Yi et al. (2018) Tertiary structure of apolipoprotein A-I in nascent high-density lipoproteins. Proc Natl Acad Sci U S A 115:5163-5168
Melchior, John T; Walker, Ryan G; Cooke, Allison L et al. (2017) A consensus model of human apolipoprotein A-I in its monomeric and lipid-free state. Nat Struct Mol Biol 24:1093-1099
Melchior, John T; Street, Scott E; Andraski, Allison B et al. (2017) Apolipoprotein A-II alters the proteome of human lipoproteins and enhances cholesterol efflux from ABCA1. J Lipid Res 58:1374-1385