Abstract

CORE-B has resources to automatically scan, recognize and measure objects at high resolution directly from microscopic slides. It is a unique configuration of a Zeiss axioplan microscope with Ludl motorized stage, fine focus and filter changer; CCD camera; Perceptics Image Capture and Processing System; Local VAX cluster; and mass storage devices. The resource is connected to others via ethernet. Locally developed software systematically scans individual microscopic sections with automatic focusing; process images for features such as stained cell bodies, silver grains, and neuronal processes; generates montages from scanned areas of different objects; statistical analyses and representations of the data; data to LONI for alignment from serial sections. CORE-B will be linked to CORE-C to receive and process images from the confocal microscope and to CORE-E to send and receive data from the Laboratory of NeuroImaging. Projects 2, 3 and 6 will use the resource to enumerate particles like silver grains and HRP granules. Projects 4, 5 and 7 will use the resource to enumerate cells, fibers and silver grains. Project 1 will use the resource to collect images of axons for depiction.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS017763-16
Application #
6112165
Study Section
Project Start
1997-06-01
Project End
2000-05-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
16
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Tychsen, Lawrence; Wong, Agnes Ming-Fong; Burkhalter, Andreas (2004) Paucity of horizontal connections for binocular vision in V1 of naturally strabismic macaques: Cytochrome oxidase compartment specificity. J Comp Neurol 474:261-75
Dagnew, Elias; Latchamsetty, Kalyan; Erinjeri, Joseph P et al. (2003) Glutamate receptor blockade alters the development of intracortical connections in rat barrel cortex. Somatosens Mot Res 20:77-84
Boero, J; Qin, W; Cheng, J et al. (2003) Restricted neuronal expression of ubiquitous mitochondrial creatine kinase: changing patterns in development and with increased activity. Mol Cell Biochem 244:69-76
Arikan, Rasim; Blake, Nicquet M J; Erinjeri, Joseph P et al. (2002) A method to measure the effective spread of focally injected muscimol into the central nervous system with electrophysiology and light microscopy. J Neurosci Methods 118:51-7
Mosconi, T; Snider, W D; Jacquin, M F (2001) Neurotrophin receptor expression in retrogradely labeled trigeminal nociceptors--comparisons with spinal nociceptors. Somatosens Mot Res 18:312-21
Epley, K D; Shainberg, M J; Lueder, G T et al. (2001) Pediatric secondary lens implantation in the absence of capsular support. J AAPOS 5:301-6
Carmichael, S T; Wei, L; Rovainen, C M et al. (2001) New patterns of intracortical projections after focal cortical stroke. Neurobiol Dis 8:910-22
Miller, B; Blake, N M; Erinjeri, J P et al. (2001) Postnatal growth of intrinsic connections in mouse barrel cortex. J Comp Neurol 436:17-31
Scott, C; Gusdorf, G; Tychsen, L (2000) Automated cover testing for binocular misalignment in awake monkeys using spectacle- mounted liquid crystal shutters. Binocul Vis Strabismus Q 15:59-66
Stark, K L; Kaufman, B; Lee, B C et al. (1999) Visual recovery after a year of craniopharyngioma-related amaurosis: report of a nine-year-old child and a review of pathophysiologic mechanisms. J AAPOS 3:366-71

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