The general objective of this Core is to provide a facility behavioral effects of genetic, pharmacological, and surgical manipulations in mice and rats tat are recovering from stroke or cardiac arrest/CPR. Behavioral examination is an integral part of the post-ischemic recovery period and is correlated with physiological data and histological end-points for each animal. This is a novel approach in the field of experimental cerebral ischemia and cardiac arrest/CPR because long term behavioral outcomes are rarely examined. Most work has emphasized histological and morphological measures of injury (or recovery). Further, in some cases, behavioral changes may be the most salient phenotypic expression observed among mutant mice.
In Aims 1 and 2, the behavioral consequences of sigma- receptor activation are assessed in normal and post-ischemic rats and in PPBP treated mice, as well as mice genetically deficient in neuronal nitric oxide synthase.
In Aims 3 and 4, we will determine if neurobehavioral outcomes after experimental stroke are more favorable in female versus male rats and if stroke injury is exacerbated in transgenic mice deficient in estrogen receptors (estrogen receptor knock-outs). Lastly, cognitive dysfunction after cardiac arrest/CPR is evaluated in Aim 5 and 3 transgenic mouse strains as a function of neuronal cytotoxicity. The Neurobehavioral Core will utilize the animals obtained from each project to gain new insight into the functional vulnerability of specific brain regions to ischemic injury and the potential for plasticity in recovery of sensory motor function, memory, and simple cognitive tasks.

Project Start
2001-12-01
Project End
2002-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
19
Fiscal Year
2002
Total Cost
$255,866
Indirect Cost
Name
Johns Hopkins University
Department
Type
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Fonken, Laura K; Gaudet, Andrew D; Gaier, Kristopher R et al. (2016) MicroRNA-155 deletion reduces anxiety- and depressive-like behaviors in mice. Psychoneuroendocrinology 63:362-9
Ni, Xinli; Yang, Zeng-Jin; Wang, Bing et al. (2012) Early antioxidant treatment and delayed hypothermia after hypoxia-ischemia have no additive neuroprotection in newborn pigs. Anesth Analg 115:627-37
Ni, Xinli; Yang, Zeng-Jin; Carter, Erin L et al. (2011) Striatal neuroprotection from neonatal hypoxia-ischemia in piglets by antioxidant treatment with EUK-134 or edaravone. Dev Neurosci 33:299-311
Woodworth, K Nina; Palmateer, Julie; Swide, Joseph et al. (2011) Short- and long-term behavioral effects of exposure to 21%, 40% and 100% oxygen after perinatal hypoxia-ischemia in the rat. Int J Dev Neurosci 29:629-38
Yang, Sufang; Abrahams, Matthew S; Hurn, Patricia D et al. (2011) Local anesthetic Schwann cell toxicity is time and concentration dependent. Reg Anesth Pain Med 36:444-51
Nakano, Takaaki; Hurn, Patricia D; Herson, Paco S et al. (2010) Testosterone exacerbates neuronal damage following cardiac arrest and cardiopulmonary resuscitation in mouse. Brain Res 1357:124-30
Yang, Zeng-Jin; Carter, Erin L; Torbey, Michel T et al. (2010) Sigma receptor ligand 4-phenyl-1-(4-phenylbutyl)-piperidine modulates neuronal nitric oxide synthase/postsynaptic density-95 coupling mechanisms and protects against neonatal ischemic degeneration of striatal neurons. Exp Neurol 221:166-74
Ohata, Hiroto; Gebremedhin, Debebe; Narayanan, Jayashree et al. (2010) Onset of pulmonary ventilation in fetal sheep produces pial arteriolar constriction dependent on cytochrome p450 omega-hydroxylase activity. J Appl Physiol 109:412-7
Zhu, W; Wang, L; Zhang, L et al. (2010) Isoflurane preconditioning neuroprotection in experimental focal stroke is androgen-dependent in male mice. Neuroscience 169:758-69
Zhang, Dandan; Hathi, Manan; Yang, Zeng-Jin et al. (2009) Hypoxic-ischemic brain injury in neonatal piglets with different histological outcomes: An amplitude-integrated EEG study. Conf Proc IEEE Eng Med Biol Soc 2009:1127-30

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