A long-standing question is how fiber pathways in the mammalian CNS diverge to both sides of the brain. Studies from our laboratory with single-fiber dye labeling have demonstrated that retinal axons from each eye distribute to both sides of the brain by diverging near the midline of the optic chiasm, a classic example of a region where fibers from two bilateral sources meet and redistribute. Immunocytochemistry with antisera to immature glia reveals a palisade of radial glia on either side of the chiasm midline. The palisade spans the region that appears to be permissive for the growth of crossed fibers, but inhibitory to the advance of uncrossed retinal fibers. The cellular structure of the chiasm midline during the period of axon outgrowth and its antigenic profile is remarkably similar to the floor plate of the spinal cord, strengthening the notion that the chiasm midline plays a role in axon guidance in the forebrain where the floor plate is absent. This proposal seeks to identify further the cellular components of the chiasm midline and their antigenic profiles, to describe the development of the chiasm and the underlying mesoderm, and to compare these features with those of the spinal cord floor plate. Finally, genes specific for the floor plate, discussed in Projects 1 and 2, will be localized in the developing chiasm. By studying the composition and development of the optic chiasm midline and the spinal cord floor plate in parallel, we will gain insight on cell patterning and the establishment of neural pathways, especially those that cross the neuraxis.

Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Rockefeller University
Department
Type
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
Ibanez-Tallon, Ines; Miwa, Julie M; Wang, Hai Long et al. (2002) Novel modulation of neuronal nicotinic acetylcholine receptors by association with the endogenous prototoxin lynx1. Neuron 33:893-903
Plump, Andrew S; Erskine, Lynda; Sabatier, Christelle et al. (2002) Slit1 and Slit2 cooperate to prevent premature midline crossing of retinal axons in the mouse visual system. Neuron 33:219-32
Ibanez-Tallon, Ines; Gorokhova, Svetlana; Heintz, Nathaniel (2002) Loss of function of axonemal dynein Mdnah5 causes primary ciliary dyskinesia and hydrocephalus. Hum Mol Genet 11:715-21
Espinosa, F; McMahon, A; Chan, E et al. (2001) Alcohol hypersensitivity, increased locomotion, and spontaneous myoclonus in mice lacking the potassium channels Kv3.1 and Kv3.3. J Neurosci 21:6657-65
Bhatt, R S; Tomoda, T; Fang, Y et al. (2000) Discoidin domain receptor 1 functions in axon extension of cerebellar granule neurons. Genes Dev 14:2216-28
Heintz, N (2000) Analysis of mammalian central nervous system gene expression and function using bacterial artificial chromosome-mediated transgenesis. Hum Mol Genet 9:937-43
Doughty, M L; De Jager, P L; Korsmeyer, S J et al. (2000) Neurodegeneration in Lurcher mice occurs via multiple cell death pathways. J Neurosci 20:3687-94
Mason, C; Erskine, L (2000) Growth cone form, behavior, and interactions in vivo: retinal axon pathfinding as a model. J Neurobiol 44:260-70
Erskine, L; Williams, S E; Brose, K et al. (2000) Retinal ganglion cell axon guidance in the mouse optic chiasm: expression and function of robos and slits. J Neurosci 20:4975-82
Miwa, J M; Ibanez-Tallon, I; Crabtree, G W et al. (1999) lynx1, an endogenous toxin-like modulator of nicotinic acetylcholine receptors in the mammalian CNS. Neuron 23:105-14

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