The purpose of this core is to provide primary human neural cells including, but not limited to, astrocytes, microglia, endothelial cells and monocytes, to all investigators within the program project. Such cells will be isolated, inventoried then shipped to investigators on a monthly basis and as needed in support of all projects within the application. Importantly, both adult and fetal sources of the neural cells will be utilized. In addition, the SCID mouse model for HIV encephalitis will be used to test both direct and indirect hypothesis for HIV associated dementia. This will include the inoculation of virus-infected astrocytes, comparisons between microglia, monocyte and/or astrocytes infected with a variety of chimeric clones of NL4-3, N-HIV-1N env, and others. Lastly, specific drugs that inhibit components of the viral life cycle and/or inflammatory products from HIV-infected and immune-activated monocytes and microglia will be investigated.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
3P01NS031492-06A1S1
Application #
6296965
Study Section
Project Start
1998-05-01
Project End
1999-04-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost
Name
St. Luke's-Roosevelt Institute for Health Sciences
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10019
Schutt, Charles R; Gendelman, Howard E; Mosley, R Lee (2018) Tolerogenic bone marrow-derived dendritic cells induce neuroprotective regulatory T cells in a model of Parkinson's disease. Mol Neurodegener 13:26
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Thomas, Midhun B; Gnanadhas, Divya Prakash; Dash, Prasanta K et al. (2018) Modulating cellular autophagy for controlled antiretroviral drug release. Nanomedicine (Lond) 13:2139-2154
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Sillman, Brady; Woldstad, Christopher; Mcmillan, Joellyn et al. (2018) Neuropathogenesis of human immunodeficiency virus infection. Handb Clin Neurol 152:21-40
Zhou, Tian; Su, Hang; Dash, Prasanta et al. (2018) Creation of a nanoformulated cabotegravir prodrug with improved antiretroviral profiles. Biomaterials 151:53-65
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AraĆ­nga, Mariluz; Edagwa, Benson; Mosley, R Lee et al. (2017) A mature macrophage is a principal HIV-1 cellular reservoir in humanized mice after treatment with long acting antiretroviral therapy. Retrovirology 14:17
Gnanadhas, Divya Prakash; Dash, Prasanta K; Sillman, Brady et al. (2017) Autophagy facilitates macrophage depots of sustained-release nanoformulated antiretroviral drugs. J Clin Invest 127:857-873

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