Abstract

The Animal core will provide investigators in projects 1 and 2 with animals prepared using models of focal and global ischemia in rats and mice, and to maintain a battery of behavioral tests for examining promising pharmacological treatments that are derived for the program project studies. An MCA occlusion model has been characterized by this group in the past, and will be used here with varying levels of severity. A global ischemia model has also been established in rats and mice. Finally, a neonatal hypoxia-ischemia model with unilateral damage will also be employed. Preclinical behavioral testing includes three sensorimotor tests: rearing, ramp walking, and rod walking. Spatial learning and memory will be tested using several measures derived from the Morris Water Maze test.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS032636-08
Application #
6661573
Study Section
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
8
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Guilliams, Kristin P; Fields, Melanie E; Ragan, Dustin K et al. (2017) Large-Vessel Vasculopathy in Children With Sickle Cell Disease: A Magnetic Resonance Imaging Study of Infarct Topography and Focal Atrophy. Pediatr Neurol 69:49-57
Murata, Takahiro; Dietrich, Hans H; Horiuchi, Tetsuyoshi et al. (2016) Mechanisms of magnesium-induced vasodilation in cerebral penetrating arterioles. Neurosci Res 107:57-62
Becker, April M; Meyers, Eric; Sloan, Andrew et al. (2016) An automated task for the training and assessment of distal forelimb function in a mouse model of ischemic stroke. J Neurosci Methods 258:16-23
Osei-Owusu, Patrick; Knutsen, Russell H; Kozel, Beth A et al. (2014) Altered reactivity of resistance vasculature contributes to hypertension in elastin insufficiency. Am J Physiol Heart Circ Physiol 306:H654-66
Hyrc, Krzysztof L; Minta, Akwasi; Escamilla, P Rogelio et al. (2013) Synthesis and properties of Asante Calcium Red--a novel family of long excitation wavelength calcium indicators. Cell Calcium 54:320-33
Shen, Hua; Hyrc, Krzysztof L; Goldberg, Mark P (2013) Maintaining energy homeostasis is an essential component of Wld(S)-mediated axon protection. Neurobiol Dis 59:69-79
Murata, Takahiro; Dietrich, Hans H; Xiang, Chuanxi et al. (2013) G protein-coupled estrogen receptor agonist improves cerebral microvascular function after hypoxia/reoxygenation injury in male and female rats. Stroke 44:779-85
Kraft, Andrew W; Hu, Xiaoyan; Yoon, Hyejin et al. (2013) Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice. FASEB J 27:187-98
Xiao, Qingli; Ford, Andria L; Xu, Jan et al. (2012) Bcl-x pre-mRNA splicing regulates brain injury after neonatal hypoxia-ischemia. J Neurosci 32:13587-96
Wacker, Bradley K; Perfater, Jennifer L; Gidday, Jeffrey M (2012) Hypoxic preconditioning induces stroke tolerance in mice via a cascading HIF, sphingosine kinase, and CCL2 signaling pathway. J Neurochem 123:954-62

Showing the most recent 10 out of 122 publications