Abstract

As in the original application, Core D will provide the histological and technical support for quantitative assessment of anatomical structures utilizing unbiased, design-based stereological techniques. Utilization of unbiased stereological counting methods will allow the investigators to ascertain accurate, quantitative data with mathematically sound methodologies. The Core will use a microscope linked with a computer and automatic stage designed to allow for optimal utilization of stereological principles to quantify parameters such as brain regional volume, cell or synapse number, and neuronal process density/length and how these parameters are modified by hypoxia-ischemia. The Core will assist in tissue processing as well as in stereological analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS032636-08
Application #
6661575
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
8
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Guilliams, Kristin P; Fields, Melanie E; Ragan, Dustin K et al. (2017) Large-Vessel Vasculopathy in Children With Sickle Cell Disease: A Magnetic Resonance Imaging Study of Infarct Topography and Focal Atrophy. Pediatr Neurol 69:49-57
Murata, Takahiro; Dietrich, Hans H; Horiuchi, Tetsuyoshi et al. (2016) Mechanisms of magnesium-induced vasodilation in cerebral penetrating arterioles. Neurosci Res 107:57-62
Becker, April M; Meyers, Eric; Sloan, Andrew et al. (2016) An automated task for the training and assessment of distal forelimb function in a mouse model of ischemic stroke. J Neurosci Methods 258:16-23
Osei-Owusu, Patrick; Knutsen, Russell H; Kozel, Beth A et al. (2014) Altered reactivity of resistance vasculature contributes to hypertension in elastin insufficiency. Am J Physiol Heart Circ Physiol 306:H654-66
Hyrc, Krzysztof L; Minta, Akwasi; Escamilla, P Rogelio et al. (2013) Synthesis and properties of Asante Calcium Red--a novel family of long excitation wavelength calcium indicators. Cell Calcium 54:320-33
Shen, Hua; Hyrc, Krzysztof L; Goldberg, Mark P (2013) Maintaining energy homeostasis is an essential component of Wld(S)-mediated axon protection. Neurobiol Dis 59:69-79
Murata, Takahiro; Dietrich, Hans H; Xiang, Chuanxi et al. (2013) G protein-coupled estrogen receptor agonist improves cerebral microvascular function after hypoxia/reoxygenation injury in male and female rats. Stroke 44:779-85
Kraft, Andrew W; Hu, Xiaoyan; Yoon, Hyejin et al. (2013) Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice. FASEB J 27:187-98
Xiao, Qingli; Ford, Andria L; Xu, Jan et al. (2012) Bcl-x pre-mRNA splicing regulates brain injury after neonatal hypoxia-ischemia. J Neurosci 32:13587-96
Wacker, Bradley K; Perfater, Jennifer L; Gidday, Jeffrey M (2012) Hypoxic preconditioning induces stroke tolerance in mice via a cascading HIF, sphingosine kinase, and CCL2 signaling pathway. J Neurochem 123:954-62

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