Abstract

The Animal Model Core will provide technical support and conduct in vivo experiments employing rodent stroke models proposed in Projects 1-3 in this program project (PPG). The main functions of the animal core can be divided into three aims: (1) To maintain the adult focal ischemia model in mice for in vivo studies, and to provide brains subjected to focal ischemia for biochemical, immunohistochemical, and/or morphological analysis.;(2) To implement and execute in vivo experimental protocols proposed by PPG investigators, including pharmacological delivery of drugs, delivery of virus for gene transfer experiments, and physiological monitoring before, during, and after ischemia;and (3) To assist and/or carry out measures of outcome including infarct volume, cerebral blood flow, blood-brain-barrier (BBB) function, and leukocyte adhesion.

Public Health Relevance

TO PUBLIC HEALTH: The Animal Model Core will support the projects in this PPG by enabling investigators to test hypotheses generated by cell culture experiments in animal models of ischemic stroke. In addition the focal ischemia model may be used to test potential therapeutic agents in preclinical studies, prior to trials in man.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS032636-15
Application #
8098711
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
2013-06-30
Budget Start
2010-07-01
Budget End
2013-06-30
Support Year
15
Fiscal Year
2010
Total Cost
$188,309
Indirect Cost

  Institution

Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Guilliams, Kristin P; Fields, Melanie E; Ragan, Dustin K et al. (2017) Large-Vessel Vasculopathy in Children With Sickle Cell Disease: A Magnetic Resonance Imaging Study of Infarct Topography and Focal Atrophy. Pediatr Neurol 69:49-57
Murata, Takahiro; Dietrich, Hans H; Horiuchi, Tetsuyoshi et al. (2016) Mechanisms of magnesium-induced vasodilation in cerebral penetrating arterioles. Neurosci Res 107:57-62
Becker, April M; Meyers, Eric; Sloan, Andrew et al. (2016) An automated task for the training and assessment of distal forelimb function in a mouse model of ischemic stroke. J Neurosci Methods 258:16-23
Osei-Owusu, Patrick; Knutsen, Russell H; Kozel, Beth A et al. (2014) Altered reactivity of resistance vasculature contributes to hypertension in elastin insufficiency. Am J Physiol Heart Circ Physiol 306:H654-66
Hyrc, Krzysztof L; Minta, Akwasi; Escamilla, P Rogelio et al. (2013) Synthesis and properties of Asante Calcium Red--a novel family of long excitation wavelength calcium indicators. Cell Calcium 54:320-33
Shen, Hua; Hyrc, Krzysztof L; Goldberg, Mark P (2013) Maintaining energy homeostasis is an essential component of Wld(S)-mediated axon protection. Neurobiol Dis 59:69-79
Murata, Takahiro; Dietrich, Hans H; Xiang, Chuanxi et al. (2013) G protein-coupled estrogen receptor agonist improves cerebral microvascular function after hypoxia/reoxygenation injury in male and female rats. Stroke 44:779-85
Kraft, Andrew W; Hu, Xiaoyan; Yoon, Hyejin et al. (2013) Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice. FASEB J 27:187-98
Xiao, Qingli; Ford, Andria L; Xu, Jan et al. (2012) Bcl-x pre-mRNA splicing regulates brain injury after neonatal hypoxia-ischemia. J Neurosci 32:13587-96
Wacker, Bradley K; Perfater, Jennifer L; Gidday, Jeffrey M (2012) Hypoxic preconditioning induces stroke tolerance in mice via a cascading HIF, sphingosine kinase, and CCL2 signaling pathway. J Neurochem 123:954-62

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