Abstract

All latency studies in mice conducted by Drs. Knipe, Coen and Schaffer and personnel in their laboratories (Projects 1, 2 and 3) will be carried out in the Redstone Facility at the Dana-Farber Cancer Institute. A shared Core Mouse Facility has been established for this purpose. The ability to support this facility as a core unit has not only proven cost-effective, but has also served to ensure uniform mouse maintenance conditions, avoid duplication of critical control tests, and minimize waste. In order to ensure that the mouse inoculation, ganglion extirpation and cocultivation procedures used are uniform, common protocols will be used by investigators on all three projects as in the past funding period. These protocols are described in section F. of Project 3 and in Appendix 1. The Core Mouse Facility will be run by Todd Astor under the direction of Drs. Schaffer, Knipe and Coen and supervisory personnel at the Redstone Facility. The Redstone Building is located adjacent to the Dana Building in which Dr. Schaffer's laboratory resides. The Redstone Facility operates in compliance with NIH and AAALAC regulations and is AAALAC accredited.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS035138-13
Application #
6112598
Study Section
Project Start
1999-03-01
Project End
2000-02-29
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
13
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Lutz, Gabriel; Jurak, Igor; Kim, Eui Tae et al. (2017) Viral Ubiquitin Ligase Stimulates Selective Host MicroRNA Expression by Targeting ZEB Transcriptional Repressors. Viruses 9:
Pan, Dongli; Kaye, Stephen B; Hopkins, Mark et al. (2014) Common and new acyclovir resistant herpes simplex virus-1 mutants causing bilateral recurrent herpetic keratitis in an immunocompetent patient. J Infect Dis 209:345-9
Pan, Dongli; Flores, Omar; Umbach, Jennifer L et al. (2014) A neuron-specific host microRNA targets herpes simplex virus-1 ICP0 expression and promotes latency. Cell Host Microbe 15:446-56
Jurak, Igor; Hackenberg, Michael; Kim, Ju Youn et al. (2014) Expression of herpes simplex virus 1 microRNAs in cell culture models of quiescent and latent infection. J Virol 88:2337-9
Wang, Jennifer P; Bowen, Glennice N; Zhou, Shenghua et al. (2012) Role of specific innate immune responses in herpes simplex virus infection of the central nervous system. J Virol 86:2273-81
Jurak, Igor; Griffiths, Anthony; Coen, Donald M (2011) Mammalian alphaherpesvirus miRNAs. Biochim Biophys Acta 1809:641-53
Kramer, Martha F; Jurak, Igor; Pesola, Jean M et al. (2011) Herpes simplex virus 1 microRNAs expressed abundantly during latent infection are not essential for latency in mouse trigeminal ganglia. Virology 417:239-47
Kramer, Martha F (2011) Stem-loop RT-qPCR for miRNAs. Curr Protoc Mol Biol Chapter 15:Unit 15.10
Jurak, Igor; Kramer, Martha F; Mellor, Joseph C et al. (2010) Numerous conserved and divergent microRNAs expressed by herpes simplex viruses 1 and 2. J Virol 84:4659-72
Kushnir, Anna S; Davido, David J; Schaffer, Priscilla A (2010) Role of nuclear factor Y in stress-induced activation of the herpes simplex virus type 1 ICP0 promoter. J Virol 84:188-200

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