Abstract

The vector core will produce herpes simplex virus (HSV)-based amplicon and retroviral vectors to be used in Projects by Giffard and Steinberg. Vectors will be produced expressing the genes for CuZn-superoxide dismutase (SOD1), manganese SOD (SOD2), glutathione peroxidase (GPX), combinations of the antioxidant genes, p35 and crmA and bcl-2. All the vectors will be """"""""bicistronic,"""""""" expressing both the gene under investigation and a reporter gene. For each experimental vector, the cognate control vector will contain the gene in question with a stop codon inserted in its center, insuring non-expression. Retroviral vectors to express SOD1, SOD2, catalase, GPx and combinations of the antioxidant genes will be produced. Control vectors will express either the reporter gene beta-galactosidase or a stop codon version of the gene of interest. The continuing purposes of the Vector Core are to ensure a constant supply of vectors for the research groups, insure quality control in the production of the vectors, and to troubleshoot problems encountered in the use of the vectors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS037520-09
Application #
7405349
Study Section
Special Emphasis Panel (ZNS1)
Project Start
Project End
Budget Start
2007-03-01
Budget End
2008-02-29
Support Year
9
Fiscal Year
2007
Total Cost
$191,825
Indirect Cost

  Institution

Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Knowland, Daniel; Arac, Ahmet; Sekiguchi, Kohei J et al. (2014) Stepwise recruitment of transcellular and paracellular pathways underlies blood-brain barrier breakdown in stroke. Neuron 82:603-17
Arac, Ahmet; Grimbaldeston, Michele A; Nepomuceno, Andrew R B et al. (2014) Evidence that meningeal mast cells can worsen stroke pathology in mice. Am J Pathol 184:2493-504
Daadi, Marcel M; Hu, Shijun; Klausner, Jill et al. (2013) Imaging neural stem cell graft-induced structural repair in stroke. Cell Transplant 22:881-92
Cheng, Michelle Y; Lee, Alex G; Culbertson, Collin et al. (2012) Prokineticin 2 is an endangering mediator of cerebral ischemic injury. Proc Natl Acad Sci U S A 109:5475-80
Horie, Nobutaka; Pereira, Marta P; Niizuma, Kuniyasu et al. (2011) Transplanted stem cell-secreted vascular endothelial growth factor effects poststroke recovery, inflammation, and vascular repair. Stem Cells 29:274-85
Andres, Robert H; Horie, Nobutaka; Slikker, William et al. (2011) Human neural stem cells enhance structural plasticity and axonal transport in the ischaemic brain. Brain 134:1777-89
Andres, Robert H; Choi, Raymond; Pendharkar, Arjun V et al. (2011) The CCR2/CCL2 interaction mediates the transendothelial recruitment of intravascularly delivered neural stem cells to the ischemic brain. Stroke 42:2923-31
Cheng, Michelle Y; Lee, I-Ping; Jin, Michael et al. (2011) An insult-inducible vector system activated by hypoxia and oxidative stress for neuronal gene therapy. Transl Stroke Res 2:92-100
Arac, Ahmet; Brownell, Sara E; Rothbard, Jonathan B et al. (2011) Systemic augmentation of alphaB-crystallin provides therapeutic benefit twelve hours post-stroke onset via immune modulation. Proc Natl Acad Sci U S A 108:13287-92
Encarnacion, Angelo; Horie, Nobutaka; Keren-Gill, Hadar et al. (2011) Long-term behavioral assessment of function in an experimental model for ischemic stroke. J Neurosci Methods 196:247-57

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