Abstract

The immunopathology core will provide investigators within the Program Project with the ability to correlate functional studies with in situ morphological analysis of the types of cells and cytokines involved in local inflammation and immune response. 1) The following specialized techniques are available through the core facility. a) tissue processing and embedding b) cryosectioning: light microscopic and electron microscopic c) immunohistochemistry d) immunofluorescence staining and microscopy e) immunofluorescence staining and microscopy f) photography 2) The aforementioned techniques will be used to: a) examine cytokine regulation in the gut and brain in autoimmune disease b) correlate the effect of stimulation with various antigenetic peptides with changes in cytokine expression in gut associated lymphoid tissue and the central nervous system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS038037-02
Application #
6410663
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
2000-12-01
Project End
2001-11-30
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
$295,873
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Kitz, Alexandra; de Marcken, Marine; Gautron, Anne-Sophie et al. (2016) AKT isoforms modulate Th1-like Treg generation and function in human autoimmune disease. EMBO Rep 17:1169-83
Murugaiyan, Gopal; da Cunha, Andre Pires; Ajay, Amrendra K et al. (2015) MicroRNA-21 promotes Th17 differentiation and mediates experimental autoimmune encephalomyelitis. J Clin Invest 125:1069-80
Zhu, Chen; Sakuishi, Kaori; Xiao, Sheng et al. (2015) An IL-27/NFIL3 signalling axis drives Tim-3 and IL-10 expression and T-cell dysfunction. Nat Commun 6:6072
Van Haren, Keith; Tomooka, Beren H; Kidd, Brian A et al. (2013) Serum autoantibodies to myelin peptides distinguish acute disseminated encephalomyelitis from relapsing-remitting multiple sclerosis. Mult Scler 19:1726-33
Wu, Chuan; Pot, Caroline; Apetoh, Lionel et al. (2013) Metallothioneins negatively regulate IL-27-induced type 1 regulatory T-cell differentiation. Proc Natl Acad Sci U S A 110:7802-7
Rangachari, Manu; Kuchroo, Vijay K (2013) Using EAE to better understand principles of immune function and autoimmune pathology. J Autoimmun 45:31-9
Liu, Sue M; Sutherland, Andrew P R; Zhang, Zheng et al. (2012) Overexpression of the Ctla-4 isoform lacking exons 2 and 3 causes autoimmunity. J Immunol 188:155-62
Lozano, Ester; Dominguez-Villar, Margarita; Kuchroo, Vijay et al. (2012) The TIGIT/CD226 axis regulates human T cell function. J Immunol 188:3869-75
Mittal, Akanksha; Murugaiyan, Gopal; Beynon, Vanessa et al. (2012) IL-27 induction of IL-21 from human CD8+ T cells induces granzyme B in an autocrine manner. Immunol Cell Biol 90:831-5
Rangachari, Manu; Zhu, Chen; Sakuishi, Kaori et al. (2012) Bat3 promotes T cell responses and autoimmunity by repressing Tim-3–mediated cell death and exhaustion. Nat Med 18:1394-400

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