Proteomics Core

Gendelman, Howard Eliot

Abstract

The purpose of this core is to provide 'state of the art' proteomic support for projects 1, 3 and 4, Drs. J. Zheng, T. Ikezu and H. Gendelman. Biomarkers will be characterized, including known and novel secretory factors produced from macrophages, microglia and/or neurons that contribute to neurodegenerative and/or neuroregenerative activities during disease. Specifically, biological fluids obtained from conditioned media or brain tissue in HIV-1 encephalitis severe combined immunodeficiency disease (SCID) and the Alzheimer's Disease (AD) mice will be evaluated. Proteins contained within samples will be identified (semi-quantitatively) and its chemical profile deciphered by Ciphergen's ProteinChip technology utilizing commercially produced chip arrays. These chips will contain a variety of surface coatings, includinghydrophobic, hydrophilic, anion, cation, metal binding and preactivated surfaces to enhance detection. Once the target biomarker has been identified, the target protein will be purified using the Amersham Pharmacia Biotech AKTA fast protein liquid chromatography (FPLC) system. The Center for Neurovirology and Neurodegenerative Disorders (CNND) has a Proteomics Core for its exclusive use. This facility houses both a complete Ciphergen's ProteinChip and a Amersham Pharmacia Biotech, AKTA FPLC system. In addition to these one- and two-dimensional (1- and 2-D) gel electrophoresis and a high performance liquid chromatography (HPLC) facilities are within the core facility.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Neurological Disorders and Stroke (NINDS)
• Type: Research Program Projects (P01)
• Project #: 5P01NS043985-04
• Application #: 7553717
• Study Section: Special Emphasis Panel (ZNS1)
• Budget Start: 2006-05-01
• Budget End: 2007-04-30
• Support Year: 4
• Fiscal Year: 2006
• Total Cost: $199,945

Institution

• Name: University of Nebraska Medical Center
• DUNS #: 168559177
• City: Omaha
• State: NE
• Country: United States
• Zip Code: 68198

Publications

Kiyota, Tomomi; Machhi, Jatin; Lu, Yaman et al. (2018) Granulocyte-macrophage colony-stimulating factor neuroprotective activities in Alzheimer's disease mice. J Neuroimmunol 319:80-92

McMillan, JoEllyn; Szlachetka, Adam; Zhou, Tian et al. (2018) Pharmacokinetic testing of a first generation cabotegravir prodrug in rhesus macaques. AIDS :

Olson, Katherine E; Bade, Aditya N; Namminga, Krista L et al. (2018) Persistent EcoHIV infection induces nigral degeneration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-intoxicated mice. J Neurovirol 24:398-410

Schutt, Charles R; Gendelman, Howard E; Mosley, R Lee (2018) Tolerogenic bone marrow-derived dendritic cells induce neuroprotective regulatory T cells in a model of Parkinson's disease. Mol Neurodegener 13:26

Thomas, Midhun B; Gnanadhas, Divya Prakash; Dash, Prasanta K et al. (2018) Modulating cellular autophagy for controlled antiretroviral drug release. Nanomedicine (Lond) 13:2139-2154

Kiyota, Tomomi; Machhi, Jatin; Lu, Yaman et al. (2018) URMC-099 facilitates amyloid-? clearance in a murine model of Alzheimer's disease. J Neuroinflammation 15:137

Kevadiya, Bhavesh D; Woldstad, Christopher; Ottemann, Brendan M et al. (2018) Multimodal Theranostic Nanoformulations Permit Magnetic Resonance Bioimaging of Antiretroviral Drug Particle Tissue-Cell Biodistribution. Theranostics 8:256-276

McMillan, JoEllyn; Szlachetka, Adam; Slack, Lara et al. (2018) Pharmacokinetics of a Long-Acting Nanoformulated Dolutegravir Prodrug in Rhesus Macaques. Antimicrob Agents Chemother 62:

Sillman, Brady; Woldstad, Christopher; Mcmillan, Joellyn et al. (2018) Neuropathogenesis of human immunodeficiency virus infection. Handb Clin Neurol 152:21-40

Zhou, Tian; Su, Hang; Dash, Prasanta et al. (2018) Creation of a nanoformulated cabotegravir prodrug with improved antiretroviral profiles. Biomaterials 151:53-65