Chronic hypoxia in children that survive premature birth leads to brain atrophy, presumably due to death of neurons and glia and altered proliferation and differenfiation of their progenitors. However, structural MRI studies also indicate that there is progressive recovery of cortical gray matter volume in these children as well as an overgrowth of gray matter in specific cortical regions. In addition, funcfional MRI studies also suggest intriguing changes in cortical representafion of language. Both results suggest that the developing brain can undergo extensive reorganization in cortical connectivity, possibly adaptive in nature. To investigate the mechanisms and the implicafions of these events, we used our hypoxic mouse model, which reproduces the initial brain atrophy and the subsequent recovery, and reared these mice in enriched environment. We will use a multidisciplinary approach to investigate whether cortical connections change after an eariy postnatal hypoxic insult and the contribution of adaptive changes in mitochondrial bioenergefics to these events. We identified and characterized mitochondrial uncoupling protein 2 (UCP2), as a crifical mitochondrial protein that enables adaptation and survival of neurons under cellular stress, including that triggered by eariy postnatal hypoxia. UCP2 promotes mitochondrial biogenesis, suppresses intracellular free radical levels, increases synaptogenesis and promotes survival of cell under hypoxia and ischemia. UCP2 was also found crifical for the metabolic adaptafion of neurons during environmental enrichment, whereby shifts in mitochondrial bioenergetics favored cellular survival and synaptic plasticity. Our central hypothesis is that improved mitochondrial bioenergetics regulated by UCP2 is a key determinant of adaptive changes in neuronal development and connectivity enhancing recovery after a hypoxic event. In this proposal.
Specific Aim 1 will elucidate the profile of mitochondrial bioenergefics in the brain of pups after eariy postnatal hypoxic insult.
Specific Aim 2 will determine whether neuronal connecfions change after eariy postnatal hypoxia, and how these connections are altered by rearing the mice in enriched environment. The execution of the above specific aims will provide new insights into the pathogenesis of neuronal abnormalifies triggered by developmental hypoxia.

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Project 4 will determine the role of mitochondrial bioenergetics and its adaptafion to promote recovery after perinatal hypoxia. The result will deliver new approaches to decrease harmful effects of hypoxia on the developing brain.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Program Projects (P01)
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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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Yale University
New Haven
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Kuwahara, Go; Hashimoto, Takuya; Tsuneki, Masayuki et al. (2017) CD44 Promotes Inflammation and Extracellular Matrix Production During Arteriovenous Fistula Maturation. Arterioscler Thromb Vasc Biol 37:1147-1156
Sadaghianloo, Nirvana; Yamamoto, Kota; Bai, Hualong et al. (2017) Increased Oxidative Stress and Hypoxia Inducible Factor-1 Expression during Arteriovenous Fistula Maturation. Ann Vasc Surg 41:225-234
Jablonska, Beata; Gierdalski, Marcin; Chew, Li-Jin et al. (2016) Sirt1 regulates glial progenitor proliferation and regeneration in white matter after neonatal brain injury. Nat Commun 7:13866
Salmaso, Natalina; Dominguez, Moises; Kravitz, Jacob et al. (2015) Contribution of maternal oxygenic state to the effects of chronic postnatal hypoxia on mouse body and brain development. Neurosci Lett 604:12-7
Koch, Marco; Varela, Luis; Kim, Jae Geun et al. (2015) Hypothalamic POMC neurons promote cannabinoid-induced feeding. Nature 519:45-50
Dimou, L; Gallo, V (2015) NG2-glia and their functions in the central nervous system. Glia 63:1429-51
Zonouzi, Marzieh; Scafidi, Joseph; Li, Peijun et al. (2015) GABAergic regulation of cerebellar NG2 cell development is altered in perinatal white matter injury. Nat Neurosci 18:674-82
Hall, Michael R; Yamamoto, Kota; Protack, Clinton D et al. (2015) Temporal regulation of venous extracellular matrix components during arteriovenous fistula maturation. J Vasc Access 16:93-106
Dietrich, Marcelo O; Zimmer, Marcelo R; Bober, Jeremy et al. (2015) Hypothalamic Agrp neurons drive stereotypic behaviors beyond feeding. Cell 160:1222-32
Gallo, Vittorio; Deneen, Benjamin (2014) Glial development: the crossroads of regeneration and repair in the CNS. Neuron 83:283-308

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