Core Unit- Gnotobiology and Diagnostic Laboratory Services The core unit will serve all projects in a highly interactive and collaborative basis. Four basic types of services will be provided. 1. Technical Services for Gnotobiology- This will include germ free isolator fabrication, modification and design and set-up as required for rodents. Technical expertise and support will be provided for the design, construction, manipulation and maintenance of special HEPA filtered enclosures for housing immunocompromised dogs and primates. Decontamination and logistical support will be provided, as well as autoclaving and decontaminating equipment, food, water, and other supplies. Cesarean derivation of rodents and other special manipulation will be provided. 2. Housing for Germ free and Gnotobiotic Rodents- Routine husbandry (feeding, watering, cage changing, etc.) will be provided for all rodents in germ free isolators. Similar husbandry and care will be provided for SCID rodents on study while housed in special HEPA filtered enclosures in the Department of Biology animal facility. The core will coordinate with University Laboratory Animal Resources on animal care related activities as required. 3. Diagnostic Laboratory Services- Existing research laboratory facilities in Laboratory Animal Medicine and in Veterinary Pathobiology will provide diagnostic support. This support will characterize and assure microbial status of animals used in the studies and will survey and assure appropriate environmental conditions for germ free isolators, HEPA filtered containment enclosures, and other areas in which animals are housed. 4. Administrative Support- The core will provide administrative support to project leaders and will coordinate all animal care and use matters with appropriate university offices.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Program Projects (P01)
Project #
5P01RR012211-02
Application #
6123497
Study Section
Project Start
1999-05-15
Project End
2000-05-14
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Suter, Steven E; Gouthro, Terry A; O'Malley, Thomas et al. (2007) Marking of peripheral T-lymphocytes by retroviral transduction and transplantation of CD34+ cells in a canine X-linked severe combined immunodeficiency model. Vet Immunol Immunopathol 117:183-96
Suter, S E; Gouthro, T A; McSweeney, P A et al. (2006) Optimized transduction of canine paediatric CD34(+) cells using an MSCV-based bicistronic vector. Vet Res Commun 30:881-901
Hallman, Troy M; Peng, Min; Meade, Ray et al. (2006) The mitochondrial and kidney disease phenotypes of kd/kd mice under germfree conditions. J Autoimmun 26:1-6
Cruickshank, Sheena M; English, Nicholas R; Felsburg, Peter J et al. (2005) Characterization of colonic dendritic cells in normal and colitic mice. World J Gastroenterol 11:6338-47
Lan, Jing-Gang; Cruickshank, Sheena-Margaret; Singh, Joy-Carmelina-Indira et al. (2005) Different cytokine response of primary colonic epithelial cells to commensal bacteria. World J Gastroenterol 11:3375-84
Krakowka, Steven; Felsburg, Peter (2005) Gnotobiotics and immunopathology: the use of the gnotobiotic environment to study acquired and inherited immunodeficiency diseases. Vet Immunol Immunopathol 108:165-75
Suter, Steven E; Gouthro, Terry A; McSweeney, Peter A et al. (2004) Isolation and characterization of pediatric canine bone marrow CD34+ cells. Vet Immunol Immunopathol 101:31-47
Cruickshank, S M; McVay, L D; Baumgart, D C et al. (2004) Colonic epithelial cell mediated suppression of CD4 T cell activation. Gut 53:678-84
Ashcroft, A J; Cruickshank, S M; Croucher, P I et al. (2003) Colonic dendritic cells, intestinal inflammation, and T cell-mediated bone destruction are modulated by recombinant osteoprotegerin. Immunity 19:849-61
Felsburg, Peter J; Hartnett, Brian J; Gouthro, Terry A et al. (2003) Thymopoiesis and T cell development in common gamma chain-deficient dogs. Immunol Res 27:235-46

Showing the most recent 10 out of 14 publications