Abstract

The Yeast 2-Hybrid Core will provide support and expertise in detection of novel protein-protein interactions through Yeast 2-Hybrid screening for the COBRE research Project Leaders, as well as other faculty both at Sanford Research/USD and at regional research institutes. The core will provide several different approaches for Yeast 2-Hybrid screening, allowing for the design of a unique, customized method for screening protein-protein interactions that best meet the needs of each individual project. By centralizing these Yeast 2-Hybrid services, the core will minimize labor and cost to individual laboratories interested in performing screens as well as bring forth the yeast genetics expertise of the core director to expedite troubleshooting or manipulation of screening systems to better meet their needs. The purpose of this core is to support the goals of the COBRE Project Leaders and other research scientist seeking to uncover novel protein-protein signaling complexes.

Public Health Relevance

The Yeast 2-Hybrid Core will perform screens for protein-protein interactions using a variety of Yeast 2- Hybrid techniques, providing scientist with novel insights into mechanisms crucial for regulation of normal cellular homeostasis as well as those underlying pathogenic conditions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103620-02
Application #
8725205
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
2
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
Sanford Research/Usd
Department
Type
DUNS #
City
Sioux Falls
State
SD
Country
United States
Zip Code
57104

  Publications

Anderson, Ruthellen H; Francis, Kevin R (2018) Modeling rare diseases with induced pluripotent stem cell technology. Mol Cell Probes 40:52-59
Amatya, Christina; Radichev, Ilian A; Ellefson, Jacob et al. (2018) Self-Transducible Bimodal PDX1-FOXP3 Protein Lifts Insulin Secretion and Curbs Autoimmunity, Boosting Tregs in Type 1 Diabetic Mice. Mol Ther 26:184-198
Yao, Qingqing; Liu, Yangxi; Selvaratnam, Balaranjan et al. (2018) Mesoporous silicate nanoparticles/3D nanofibrous scaffold-mediated dual-drug delivery for bone tissue engineering. J Control Release 279:69-78
Louwagie, Eli J; Larsen, Tricia D; Wachal, Angela L et al. (2018) Placental lipid processing in response to a maternal high-fat diet and diabetes in rats. Pediatr Res 83:712-722
White, Katherine A; Swier, Vicki J; Cain, Jacob T et al. (2018) A porcine model of neurofibromatosis type 1 that mimics the human disease. JCI Insight 3:
Anderson, Ruthellen H; Lensing, Cody J; Forred, Benjamin J et al. (2018) Differentiating Antiproliferative and Chemopreventive Modes of Activity for Electron-Deficient Aryl Isothiocyanates against Human MCF-7 Cells. ChemMedChem 13:1695-1710
Johnson, Tyler B; Mechels, Keegan; Anderson, Ruth Ellen et al. (2018) Characterization of a recurrent missense mutation in the forkhead DNA-binding domain of FOXP1. Sci Rep 8:16161
Brudvig, J J; Cain, J T; Sears, R M et al. (2018) MARCKS regulates neuritogenesis and interacts with a CDC42 signaling network. Sci Rep 8:13278
Roux, Kyle J; Kim, Dae In; Burke, Brian et al. (2018) BioID: A Screen for Protein-Protein Interactions. Curr Protoc Protein Sci 91:19.23.1-19.23.15
Hussain, Sajjad; Bedekovics, Tibor; Liu, Qiuying et al. (2018) UCH-L1 bypasses mTOR to promote protein biosynthesis and is required for MYC-driven lymphomagenesis in mice. Blood 132:2564-2574

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