Abstract

The Aim of the Flow Cytometry Core is to provide access to state-of-the art flow cytometry for all the participants in this Developmental Biology COBRE. The Flow Cytometry Core has two high speed fluorescence activated cell sorters (Dako-Cytomation MoFlo and BD FACSAria) and three analyzers (FACScan, FACSCalibur and LSRll analyzer, all from BD). Dr. Jose Alberola-lla, who is an expert in multicolor analysis and sorting of hematopoietic progenitors, will serve as Core Director and technical assistance will be provided by Dr. Diana Hamilton. Access to the Flow Cytometry Core will be free of charge for all COBRE participants. The research projects of four of the Junior Investigators depend upon flow cytometry and the fifth one proposes flow cytometry as an alternate approach. Thus, this Core will be heavily used and is critical for the success of this COBRE.

Public Health Relevance

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  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
1P20GM103636-01A1
Application #
8466508
Study Section
Special Emphasis Panel (ZGM1-TWD-B (CB))
Project Start
Project End
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
1
Fiscal Year
2013
Total Cost
$84,000
Indirect Cost
$34,000

  Institution

Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
077333797
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104

  Publications

Bhaskaran, Shylesh; Pharaoh, Gavin; Ranjit, Rojina et al. (2018) Loss of mitochondrial protease ClpP protects mice from diet-induced obesity and insulin resistance. EMBO Rep 19:
Siefert, Joseph C; Clowdus, Emily A; Goins, Duane et al. (2018) Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System. J Vis Exp :
Borga, Chiara; Park, Gilseung; Foster, Clay et al. (2018) Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis. Leukemia :
Wren, Jonathan D (2018) Algorithmically outsourcing the detection of statistical errors and other problems. EMBO J 37:
Georgescu, Constantin; Wren, Jonathan D (2018) Algorithmic identification of discrepancies between published ratios and their reported confidence intervals and P-values. Bioinformatics 34:1758-1766
Snider, Timothy A; Richardson, Arlan; Stoner, Julie A et al. (2018) The Geropathology Grading Platform demonstrates that mice null for Cu/Zn-superoxide dismutase show accelerated biological aging. Geroscience 40:97-103
Sansam, Courtney G; Pietrzak, Katarzyna; Majchrzycka, Blanka et al. (2018) A mechanism for epigenetic control of DNA replication. Genes Dev 32:224-229
Siefert, Joseph C; Georgescu, Constantin; Wren, Jonathan D et al. (2017) DNA replication timing during development anticipates transcriptional programs and parallels enhancer activation. Genome Res 27:1406-1416
Jordan, Philip W; Eyster, Craig; Chen, Jingrong et al. (2017) Sororin is enriched at the central region of synapsed meiotic chromosomes. Chromosome Res 25:115-128
Giles, Cory B; Brown, Chase A; Ripperger, Michael et al. (2017) ALE: automated label extraction from GEO metadata. BMC Bioinformatics 18:509

Showing the most recent 10 out of 57 publications