Abstract

High-throughput 'omics analysis platforms are the technologies of choice for analyzing complex mixtures derived from tissues and biological matrices such as liver, feces, intestine, blood and urine. To maximize our capability for deciphering the mechanism and therapy for liver injury, nutrition and gut:liver interactions, and liver:environment/toxin/drug interactions, we propose an OMICS Core in this Hepatobiology and Toxicology COBRE proposal. Our OMICS Core is composed of three major components including Metabolomics Core, Proteomics Core, and Bioinformatics. The overarching goal of the OMICS Core is to support the proposed COBRE research. This goal will be met through application of state of the art separations science and mass spectrometry techniques to analyze research samples containing complex mixtures of metabolites, proteins and protein fragments. This goal is achieved through the following aims:
Specific Aim 1 : Application of liquid chromatography mass spectrometry (LC-MS), comprehensive two- dimensional gas chromatography time-of-flight mass spectrometry (GCxGC/TOF-MS) and integrated laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) platforms for quantitative analysis of metabolites, metal ions, and metal-protein complex in biological samples.
Specific Aim 2 : Application of high-resolution multidimensional liquid chromatography mass spectrometry (MDLC-MS) and affinity enrichment methods for targeted protein quantification.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM113226-04
Application #
9673150
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2019-04-01
Budget End
2020-03-31
Support Year
4
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
University of Louisville
Department
Type
DUNS #
057588857
City
Louisville
State
KY
Country
United States
Zip Code
40292

  Publications

Hardesty, Josiah E; Al-Eryani, Laila; Wahlang, Banrida et al. (2018) Epidermal Growth Factor Receptor Signaling Disruption by Endocrine and Metabolic Disrupting Chemicals. Toxicol Sci 162:622-634
Cui, Guozhen; Martin, Robert C; Liu, Xingkai et al. (2018) Serological biomarkers associate ultrasound characteristics of steatohepatitis in mice with liver cancer. Nutr Metab (Lond) 15:71
Cui, Guozhen; Martin, Robert C; Jin, Hang et al. (2018) Up-regulation of FGF15/19 signaling promotes hepatocellular carcinoma in the background of fatty liver. J Exp Clin Cancer Res 37:136
Zheng, Yuxuan; Ritzenthaler, Jeffrey D; Burke, Tom J et al. (2018) Age-dependent oxidation of extracellular cysteine/cystine redox state (Eh(Cys/CySS)) in mouse lung fibroblasts is mediated by a decline in Slc7a11 expression. Free Radic Biol Med 118:13-22
Hein, David W; Zhang, Xiaoyan; Doll, Mark A (2018) Role of N-acetyltransferase 2 acetylation polymorphism in 4, 4'-methylene bis (2-chloroaniline) biotransformation. Toxicol Lett 283:100-105
Hein, David W; Fakis, Giannoulis; Boukouvala, Sotiria (2018) Functional expression of human arylamine N-acetyltransferase NAT1*10 and NAT1*11 alleles: a mini review. Pharmacogenet Genomics 28:238-244
Liang, Yaqin; Lang, Anna L; Zhang, Jian et al. (2018) Exposure to Vinyl Chloride and Its Influence on Western Diet-Induced Cardiac Remodeling. Chem Res Toxicol 31:482-493
Kharbanda, Kusum K; Ronis, Martin J J; Shearn, Colin T et al. (2018) Role of Nutrition in Alcoholic Liver Disease: Summary of the Symposium at the ESBRA 2017 Congress. Biomolecules 8:
Ghosh Dastidar, Shubha; Warner, Jeffrey B; Warner, Dennis R et al. (2018) Rodent Models of Alcoholic Liver Disease: Role of Binge Ethanol Administration. Biomolecules 8:
Gosney, Julie A; Wilkey, Daniel W; Merchant, Michael L et al. (2018) Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner. J Biol Chem 293:5895-5908

Showing the most recent 10 out of 68 publications