Abstract

TheproposedUniversityofKentuckyCenterforCancerandMetabolism(CCM)isdesignedtoaddressa significantgapincancerresearchbyprovidingthemultidisciplinaryinfrastructuretosupportcareer developmentintheareaofmetabolicreprogrammingofthetumormicroenvironment.Routineandhigh-end imagingconstituteacriticalcomponentofthiseffort,astheabilitytovisualizeandquantifytargetmolecules andtheireffectsoncellularstructureandfunctionareheavilydependentontheavailabilityandqualityofthe imagingsystems.TheImagingCoreinthisapplicationwillprovidetechnicalservicesandsupportforCCM projectresearchlaboratoriesandpilotprojectrecipientsandiswellpositionedtoadvancetheCCMmissionin cancerandmetabolismthroughdeliveryofanexpandedrangeofimagingservicestoCOBREinvestigators andothers.ThiswillbeaccomplishedbytheachievementofthreeSpecificAims:
SpecificAim1. Toprovide project-specifictrainingandconsultationforCOBREinvestigators.
SpecificAim2. Todevelopand/oradapt state-of-theartimagingtechniquestoenhancetheresearchofearly-stageandpilotprojectCOBRE investigators.
SpecificAim3. Todevelopandimplementnewmethods,suchasproject-specificsample preparation,toenhanceCCMimagingservicesandmakesuchservicesavailabletoCOBREandnon-COBRE users.ImagingCoreuserswillhaveaccesstothisno-costfacilitywhereinvestigatorscanevaluatetheirtissue orcellularsamplesusingvarioustypesofinstruments.Thecorewillalsosupportroutineanalysisaswellas introduceinvestigatorstohigh-endimagingoptionsandcapabilitiesrelevanttotheirstudies,therebyfacilitating theresearchsuccessesoftheseusers.Dr.JianhangJia,theDirectoroftheImagingCore,isahighly productiveinvestigatorwhohassuccessfullyobtainedsubstantialextramuralresearchsupportthatrelies extensivelyonsophisticatedimagingcapabilities.Hehas,thus,investedextensiveeffortinthedevelopment andimprovementofinstrumentationwithintheMarkeyCancerCenter.HewillconsultwithCCMinvestigators onthedesignofexperimentsusingimagingmodalities,arolethatheassumedinguidingthedevelopmentof thefourCCMresearchprojectsinthisapplication.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM121327-02
Application #
9441817
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2018-01-01
Budget End
2018-12-31
Support Year
2
Fiscal Year
2018
Total Cost
Indirect Cost

  Institution

Name
University of Kentucky
Department
Type
DUNS #
939017877
City
Lexington
State
KY
Country
United States
Zip Code
40526

  Publications

Wang, Jianlin; Qiu, Zhaoping; Wu, Yadi (2018) Ubiquitin Regulation: The Histone Modifying Enzyme's Story. Cells 7:
Zaytseva, Yekaterina Y; Rychahou, Piotr G; Le, Anh-Thu et al. (2018) Preclinical evaluation of novel fatty acid synthase inhibitors in primary colorectal cancer cells and a patient-derived xenograft model of colorectal cancer. Oncotarget 9:24787-24800
Jiang, Kai; Liu, Yajuan; Zhang, Jie et al. (2018) An intracellular activation of Smoothened that is independent of Hedgehog stimulation in Drosophila. J Cell Sci 131:
Chaiswing, Luksana; St Clair, William H; St Clair, Daret K (2018) Redox Paradox: A Novel Approach to Therapeutics-Resistant Cancer. Antioxid Redox Signal 29:1237-1272
Chaiswing, Luksana; Weiss, Heidi L; Jayswal, Rani D et al. (2018) Profiles of Radioresistance Mechanisms in Prostate Cancer. Crit Rev Oncog 23:39-67
Jarrett, Stuart G; Carter, Katharine M; Bautista, Robert-Marlo et al. (2018) Sirtuin 1-mediated deacetylation of XPA DNA repair protein enhances its interaction with ATR protein and promotes cAMP-induced DNA repair of UV damage. J Biol Chem 293:19025-19037
Yu, Qian; Zhou, Binhua P; Wu, Yadi (2017) The regulation of snail: on the ubiquitin edge. Cancer Cell Microenviron 4:
Lin, Yiwei; Wang, Yu; Shi, Qing et al. (2017) Stabilization of the transcription factors slug and twist by the deubiquitinase dub3 is a key requirement for tumor metastasis. Oncotarget 8:75127-75140