This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of the Transgenic/Microinjection Core Facility is to provide sophisticated mammalian molecular genetic services to the investigators of this proposal, as well as other investigators at OMRF and OUHSC. Services include the production of genetically engineered mice through introduction of DNA into zygotes as well as through injection of genetically manipulated embryonic stem cells into blastocysts, and the rederivation of mouse strains through implantation of embryos into foster recipients. All microinjections and animal manipulations in these procedures are performed by the Microinjection Core Facility. Candidate transgenic pups (from zygote injections), chimeras (from blastocyst injections), and rederived pups are transferred to the requesting investigator for molecular analysis and further breeding. Applications for zygote injection are the generation of mice expressing a gene which normally is not expressed in a particular tissue or cell type, expressing mutated genes, expressing human genes in mice, or expressing additional copies of genes such as in aneuploid models. Depending on experimantal parameters, injected DNA can be in the form of a plasmid carrying the coding region of a gene under control of a specific promoter, or large genomic regions carrying their own regulatory elements in vectors such as BACs. Embryonic stem cells are used for the creation of mouse strains carrying a null allele of the gene of interest (knock-out strains), a mutated allele of a gene (knock-in strains), defined chromosomal deletions, duplications, or translocations, and strains with conditionally altered temporal and/or spatial expression of genes (conditional knock-out). Rederivation is necessary in two situations: First, when investigators need a mouse strain which currently is only distributed as frozen embryos, and second, when investigators want to import mice from collaborating laboratories and the mice carry known pathogens, necessitating removal of these pathogens through rederivation. A modern Transgenic/Microinjection Core Facility able to provide molecular genetic services adds important strengths to investigators within this COBRE proposal as well as to investigators at OMRF, OUHSC, and throughout the state of Oklahoma.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR015577-07
Application #
7381145
Study Section
Special Emphasis Panel (ZRR1-RI-8 (01))
Project Start
2006-05-01
Project End
2007-04-30
Budget Start
2006-05-01
Budget End
2007-04-30
Support Year
7
Fiscal Year
2006
Total Cost
$74,263
Indirect Cost
Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
077333797
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2018) Modeling Transcriptional Rewiring in Neutrophils Through the Course of Treated Juvenile Idiopathic Arthritis. Sci Rep 8:7805
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2016) Complexity and Specificity of the Neutrophil Transcriptomes in Juvenile Idiopathic Arthritis. Sci Rep 6:27453
Molineros, Julio E; Maiti, Amit K; Sun, Celi et al. (2013) Admixture mapping in lupus identifies multiple functional variants within IFIH1 associated with apoptosis, inflammation, and autoantibody production. PLoS Genet 9:e1003222
Smith, Kenneth; Muther, Jennifer J; Duke, Angie L et al. (2013) Fully human monoclonal antibodies from antibody secreting cells after vaccination with Pneumovax®23 are serotype specific and facilitate opsonophagocytosis. Immunobiology 218:745-54
Dozmorov, Igor; Dominguez, Nicolas; Sestak, Andrea L et al. (2013) Evidence of dynamically dysregulated gene expression pathways in hyperresponsive B cells from African American lupus patients. PLoS One 8:e71397
Jiang, Kaiyu; Frank, Mark; Chen, Yanmin et al. (2013) Genomic characterization of remission in juvenile idiopathic arthritis. Arthritis Res Ther 15:R100
Kurien, Biji T; D'Sousa, Anil; Bruner, Benjamin F et al. (2013) Prolidase deficiency breaks tolerance to lupus-associated antigens. Int J Rheum Dis 16:674-80
Lessard, Christopher J; Adrianto, Indra; Ice, John A et al. (2012) Identification of IRF8, TMEM39A, and IKZF3-ZPBP2 as susceptibility loci for systemic lupus erythematosus in a large-scale multiracial replication study. Am J Hum Genet 90:648-60
Weckerle, Corinna E; Mangale, Dorothy; Franek, Beverly S et al. (2012) Large-scale analysis of tumor necrosis factor ? levels in systemic lupus erythematosus. Arthritis Rheum 64:2947-52
James, Judith A; Robertson, Julie M (2012) Lupus and Epstein-Barr. Curr Opin Rheumatol 24:383-8

Showing the most recent 10 out of 194 publications