Abstract

It is proposed to establish a single, integrated, stand alone mouse transgenic and knockout core facility that will serve the needs of investigators at Brown, affiliated teaching hospitals, and throughout the region. This core will be the first mouse transgenic facility in the state of Rhode Island. The core will be dedicated to serve investigators in many disciplines, and aid them in the development and implementation of transgenic mouse model systems in their area of study. The core will perform pro-nuclear microinjections as well as blastocyst injections. In addition, protocols, genotype ES cell clones as well as founder animals by PCR, and maintain a mouse colony to support these efforts. The core will also support the research efforts of a magnet investigator, a nationally recognized scientist in the field of mouse transgenics, who will be hired and who will become the Director of this core. On a day to day basis the core will be run by a highly trained, Ph.D. level Associate Director who will be appointed at the rank of Assistant professor (Research).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
3P20RR015578-01S1
Application #
6419668
Study Section
Special Emphasis Panel (ZRR1)
Project Start
2000-09-30
Project End
2001-08-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2001
Total Cost
$236,556
Indirect Cost

  Institution

Name
Brown University
Department
Type
DUNS #
001785542
City
Providence
State
RI
Country
United States
Zip Code
02912

  Publications

Lovasco, Lindsay A; Gustafson, Eric A; Seymour, Kimberly A et al. (2015) TAF4b is required for mouse spermatogonial stem cell development. Stem Cells 33:1267-76
Ribeiro, Jennifer R; Freiman, Richard N (2014) Estrogen signaling crosstalk: Implications for endocrine resistance in ovarian cancer. J Steroid Biochem Mol Biol 143:160-73
Casella, Cinzia; Miller, Daniel H; Lynch, Kerry et al. (2014) Oxysterols synergize with statins by inhibiting SREBP-2 in ovarian cancer cells. Gynecol Oncol 135:333-41
Grive, Kathryn J; Seymour, Kimberly A; Mehta, Rajvi et al. (2014) TAF4b promotes mouse primordial follicle assembly and oocyte survival. Dev Biol 392:42-51
Tomimaru, Yoshito; Xu, Chelsea Q; Nambotin, Sarah B et al. (2013) Loss of exon 4 in a human T-cell factor-4 isoform promotes hepatic tumourigenicity. Liver Int 33:1536-48
Minhas, Hassan M; Pescosolido, Matthew F; Schwede, Matthew et al. (2013) An unbalanced translocation involving loss of 10q26.2 and gain of 11q25 in a pedigree with autism spectrum disorder and cerebellar juvenile pilocytic astrocytoma. Am J Med Genet A 161A:787-91
De Cecco, Marco; Criscione, Steven W; Peckham, Edward J et al. (2013) Genomes of replicatively senescent cells undergo global epigenetic changes leading to gene silencing and activation of transposable elements. Aging Cell 12:247-56
Li, Hua; Jogl, Gerwald (2013) Crystal structure of decaprenylphosphoryl-?- D-ribose 2'-epimerase from Mycobacterium smegmatis. Proteins 81:538-43
Tomimaru, Yoshito; Koga, Hironori; Yano, Hirohisa et al. (2013) Upregulation of T-cell factor-4 isoform-responsive target genes in hepatocellular carcinoma. Liver Int 33:1100-12
Tomimaru, Yoshito; Koga, Hironori; Shin, Tai Ho et al. (2013) The SxxSS motif of T-cell factor-4 isoforms modulates Wnt/?-catenin signal activation in hepatocellular carcinoma cells. Cancer Lett 336:359-69

Showing the most recent 10 out of 152 publications