Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core houses an Affymetrix GeneChip instrument, which is operated by a dedicated specialist, Carl Simkevich. He is responsible for generating probes from RNA provided by the investigators, and all subsequent aspects of hybridization and scanning. This is a fee for service facility. The Core also houses a real time PCR instrument (ABI 7700), a Becton Dickinson FACSCalibur flow cytometer, a LiCor infrared scanner, a fluorescence/luminescence microplate reader, and an Amersham Typhoon 4120 laser scanner. All instruments (with the exception of the Affymetrix GeneChip) are set up as a user-operated shared instrumentation resource. A computer core consisting of 2 Gateway workstations is available for individual investigators to analyze their own data. The workstations run all proprietary Affymetrix software as well as second vendor software such as GeneSpring and the Celera database. Carl Simkevich is available for consultation on data analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR015578-07
Application #
7381152
Study Section
Special Emphasis Panel (ZRR1-RI-8 (01))
Project Start
2006-05-01
Project End
2007-04-30
Budget Start
2006-05-01
Budget End
2007-04-30
Support Year
7
Fiscal Year
2006
Total Cost
$394,918
Indirect Cost

  Institution

Name
Brown University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
001785542
City
Providence
State
RI
Country
United States
Zip Code
02912

  Publications

Lovasco, Lindsay A; Gustafson, Eric A; Seymour, Kimberly A et al. (2015) TAF4b is required for mouse spermatogonial stem cell development. Stem Cells 33:1267-76
Ribeiro, Jennifer R; Freiman, Richard N (2014) Estrogen signaling crosstalk: Implications for endocrine resistance in ovarian cancer. J Steroid Biochem Mol Biol 143:160-73
Casella, Cinzia; Miller, Daniel H; Lynch, Kerry et al. (2014) Oxysterols synergize with statins by inhibiting SREBP-2 in ovarian cancer cells. Gynecol Oncol 135:333-41
Grive, Kathryn J; Seymour, Kimberly A; Mehta, Rajvi et al. (2014) TAF4b promotes mouse primordial follicle assembly and oocyte survival. Dev Biol 392:42-51
Tomimaru, Yoshito; Xu, Chelsea Q; Nambotin, Sarah B et al. (2013) Loss of exon 4 in a human T-cell factor-4 isoform promotes hepatic tumourigenicity. Liver Int 33:1536-48
Minhas, Hassan M; Pescosolido, Matthew F; Schwede, Matthew et al. (2013) An unbalanced translocation involving loss of 10q26.2 and gain of 11q25 in a pedigree with autism spectrum disorder and cerebellar juvenile pilocytic astrocytoma. Am J Med Genet A 161A:787-91
De Cecco, Marco; Criscione, Steven W; Peckham, Edward J et al. (2013) Genomes of replicatively senescent cells undergo global epigenetic changes leading to gene silencing and activation of transposable elements. Aging Cell 12:247-56
Li, Hua; Jogl, Gerwald (2013) Crystal structure of decaprenylphosphoryl-?- D-ribose 2'-epimerase from Mycobacterium smegmatis. Proteins 81:538-43
Tomimaru, Yoshito; Koga, Hironori; Yano, Hirohisa et al. (2013) Upregulation of T-cell factor-4 isoform-responsive target genes in hepatocellular carcinoma. Liver Int 33:1100-12
Tomimaru, Yoshito; Koga, Hironori; Shin, Tai Ho et al. (2013) The SxxSS motif of T-cell factor-4 isoforms modulates Wnt/?-catenin signal activation in hepatocellular carcinoma cells. Cancer Lett 336:359-69

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