This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The Microscopy Core Research Facility (MCRF) has been one of the most heavily used cores in Nebraska, which provides the-state-of-art instrumentation and professional services to all researchers from both academic and industrial organizations. The MCRF has two Olympus confocal laser scanning microscope systems (one with an upright scope and the other with an inverted system) with the capacity for simultaneous display of double or triple fluorescence labeled materials and two advanced fluorescence microscopes with high-sensitivity video/digital cameras. These imaging systems have been used by most of NCV members cross the UNL campus and by several members from UNMC. The MCRF Manager and staff have set up strong research collaborations with many at least 40 faulty/PIs and has been utilized by 60-85 faculty groups annually (73 PIs in this year). This resulted in several publications in which the research groups utilized the equipment supported by the NCV grant and Zhou's expertise (see list of publications). The Core has also been involved in undergraduate and graduate education/training programs and has organized demonstrations of imaging systems for ~ 200 students during the past year. In addition, the MCRF has been actively participating in grant application (such as NCV, Redox Center, and Cell Signaling, as well as many individual investigators) and has been a key component of the NCV.
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