This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.When immune cells recognize the presence of a pathogen, they receive signals that cause the cell to respond to the pathogen. We are studying the signaling pathways in one type of immune cells called T cells. In order for a T cell to respond to a pathogen, it must receive signals from two receptors: the T cell receptor (TCR) and a co-receptor such as CD28. Although it is unknown that signals from two receptors are required to activate T cells, the mechanisms by which the signals are coordinated remain unclear. The goal of this research project is to examine how the signaling pathways initiated by the TCR and CD28 combine to result in the activation of T cells. Our research has focused on the Gads adaptor protein, a signaling protein required for optimal TCR-mediated signaling. We previously generated a Gads-deficient mouse line and found that Gads is required for several stages during T cell development. Comparing our mouse line to reports in the literature describing CD28-/- mice, we found strikingly similar phenotypes. This led to the hypothesis that Gads might regulate CD28-mediated signaling. Specifically, we hypothesize that Gads, CD28, and Akt are linked in a common signaling pathway. In this proposal, we are using biochemical and genetic techniques to investigate whether Gads can regulate CD28-mediated Akt phosphorylation.
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