This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Genetic diversity of eastern lampmussel, Lampsilis radiata, populations in Lake Champlain and its tributaries Native mussel populations are declining due to increased zebra mussel densities. As subpopulations of threatened and endangered mussels disappear, it is necessary to develop conservation strategies for the remaining populations. Conservation strategies are dependent on the population genetics of the remaining individuals. For example, genetically distinct subpopulations should probably not be supplemented with mussels from other populations. Subpopulations may represent locally adapted phenotypes and genes from other populations may reduce their fitness. If existing subpopulations regularly exchange genes under natural conditions, then transplanting individuals from other populations may be justified to bolster declining local populations. We are currently using microsatellite markers to determine the extent of gene flow between populations of Lampsilis radiata in Lake Champlain and its tributaries. Microsatellite primers have been developed by Eackles and King (2002) and we have successfully amplified these loci in Lake Champlain mussels and in mussels from the Winooski River using these primers. The information from these markers will provide measurements of gene flow between these separate subpopulations. This information will inform policy makers and biologists charged with the conservation of native Champlain-basin mussels.
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