This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The hypothesis underlying this project is that functionally important regulatory sequences will be conserved in the non-coding flanking regions of orthologous genes from distantly related organisms such as mammals and elasmobranchs such that a comparative analysis of these regions will uncover potentially novel cis-acting transcriptional regulatory sequences. We will compare 5- and 3-non-coding regions of shark and skate receptor genes with those of orthologous mammalian genes to identify putative regulatory elements that have been conserved over 450 million years. Since receptor tyrosine kinase genes are completely uncharacterized in elasmobranch species, the cloning and sequencing of full-length receptor cDNAs is an important part of this project. Full-length cDNAs are required to establish orthology with receptor kinase genes from other species.
Showing the most recent 10 out of 246 publications
