This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This year one PhD student, one masters student and a full time technician joined the lab as well as a honors undergraduate student. They have each successfully competed for INBRE funding and my PhD student is applying for external fellowships. This and purchase of an additional thermocycler increased lab productivity considerably allowing us to gather enough data to clarify and focus the CAV1/AhR research. Analyses of approximately 30 Hepa1c1c7 clones by RTPCR allowed us to identify a series of clones with increasing levels of CAV1 expression and determine that AhR-dependent gene expression (i.e., CYP1A1 induction) was enhanced by low level CAV1 expression. This effect reached a maximum in clones expressing moderate levels of CAV1 and then AhR-dependent gene expression began to decrease with additional CAV1 expression creating a bell shaped AhR-dependent gene expression curve with increasing CAV1. Immunofluorescence microscopy revealed CAV1 localization to the cytoplasm at low expression levels which switched to predominantly plasma membrane localization in high CAV1 expressing clones. This reconciles all our preliminary data and is consistent with recently published data indicating that increasing CAV1 protein results in formation of high molecular weight oligomers. As CAV1 oligomerizes in vivo, it is transported from cytosolic pools to the plasma membrane. We have evaluated endogenous CAV1 expression and localization in three additional cell lines. These results are now part of a R15 proposal being submitted May 05 and should be ready for publication late 2005. The additional lab personnel have also allowed for significant development on two additional projects that should be ready for funding/publication in 2006. As the preliminary data allows for more clarified hypotheses, I am working with NIH program staff to identify and target my proposals to appropriate funding mechanisms.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Exploratory Grants (P20)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-RI-7 (02))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Alaska Fairbanks
Organized Research Units
United States
Zip Code
Wilson, Robert E; Ely, Craig R; Talbot, Sandra L (2018) Flyway structure in the circumpolar greater white-fronted goose. Ecol Evol 8:8490-8507
Hueffer, Karsten; Khatri, Shailesh; Rideout, Shane et al. (2017) Rabies virus modifies host behaviour through a snake-toxin like region of its glycoprotein that inhibits neurotransmitter receptors in the CNS. Sci Rep 7:12818
Ely, Craig R; Wilson, Robert E; Talbot, Sandra L (2017) Genetic structure among greater white-fronted goose populations of the Pacific Flyway. Ecol Evol 7:2956-2968
Talbot, Sandra L; Sage, George K; Sonsthagen, Sarah A et al. (2017) Intraspecific evolutionary relationships among peregrine falcons in western North American high latitudes. PLoS One 12:e0188185
Leewis, Mary-Cathrine; Uhlik, Ondrej; Fraraccio, Serena et al. (2016) Differential Impacts of Willow and Mineral Fertilizer on Bacterial Communities and Biodegradation in Diesel Fuel Oil-Contaminated Soil. Front Microbiol 7:837
Harley, John R; Bammler, Theo K; Farin, Federico M et al. (2016) Using Domestic and Free-Ranging Arctic Canid Models for Environmental Molecular Toxicology Research. Environ Sci Technol 50:1990-9
Hoffman, M; Taylor, B E; Harris, M B (2016) Evolution of lung breathing from a lungless primitive vertebrate. Respir Physiol Neurobiol 224:11-6
Vayndorf, Elena M; Scerbak, Courtney; Hunter, Skyler et al. (2016) Morphological remodeling of C. elegans neurons during aging is modified by compromised protein homeostasis. NPJ Aging Mech Dis 2:
Goldsmith, Elizabeth W; Renshaw, Benjamin; Clement, Christopher J et al. (2016) Population structure of two rabies hosts relative to the known distribution of rabies virus variants in Alaska. Mol Ecol 25:675-88
Leewis, Mary-Cathrine; Uhlik, Ondrej; Leigh, Mary Beth (2016) Synergistic Processing of Biphenyl and Benzoate: Carbon Flow Through the Bacterial Community in Polychlorinated-Biphenyl-Contaminated Soil. Sci Rep 6:22145

Showing the most recent 10 out of 113 publications