This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator.
Specific Aims : To clone ChChd3, ChChd6 and Mia40 into mammalian expression vectors with either GFP tags or Flag tags for visualization in an epifluorescent microscope. To introduce recombinant ChChd3, ChChd6, and Mia40 DNA into mammalian tissue culture cells and determine localization of the wild type proteins. To use bioinformatics techniques to determine potentially important mutation sites on the proteins. To introduce mutations in ChChd3, ChChd6, and Mia40 and monitor the effect on subcellular localization of the expressed proteins. To determine which amino acids in the proteins are necessary for targeting the proteins to the mitochondria. To explore other conserved sequence motifs through mutation and the effect on the proteins. Hypothesis: Proteins containing similar coiled coil-helix domains (CHCH) have a predicted mitochondrial localization. Mutation of key amino acids in the CHCH domain will disrupt targeting of the novel CHCH domain containing proteins ChChd3 and ChChd6.
|Barta, Cody L; Liu, Huizhan; Chen, Lei et al. (2018) RNA-seq transcriptomic analysis of adult zebrafish inner ear hair cells. Sci Data 5:180005|
|Liu, Huizhan; Chen, Lei; Giffen, Kimberlee P et al. (2018) Cell-Specific Transcriptome Analysis Shows That Adult Pillar and Deiters' Cells Express Genes Encoding Machinery for Specializations of Cochlear Hair Cells. Front Mol Neurosci 11:356|
|Wehrkamp, Cody J; Natarajan, Sathish Kumar; Mohr, Ashley M et al. (2018) miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family. RNA Biol 15:391-403|
|Lopez, Wilfredo; Page, Alexis M; Carlson, Darby J et al. (2018) Analysis of immune-related genes during Nora virus infection of Drosophila melanogaster using next generation sequencing. AIMS Microbiol 4:123-139|
|Gong, Qiang; Wang, Chao; Zhang, Weiwei et al. (2017) Assessment of T-cell receptor repertoire and clonal expansion in peripheral T-cell lymphoma using RNA-seq data. Sci Rep 7:11301|
|Lu, Guoqing; Luhr, Jamie; Stoecklein, Andrew et al. (2017) Complete Genome Sequences ofPseudomonas fluorescensBacteriophages Isolated from Freshwater Samples in Omaha, Nebraska. Genome Announc 5:|
|Azadmanesh, Jahaun; Trickel, Scott R; Weiss, Kevin L et al. (2017) Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals. Acta Crystallogr F Struct Biol Commun 73:235-240|
|Bouska, A; Zhang, W; Gong, Q et al. (2017) Combined copy number and mutation analysis identifies oncogenic pathways associated with transformation of follicular lymphoma. Leukemia 31:83-91|
|Azadmanesh, Jahaun; Trickel, Scott R; Borgstahl, Gloria E O (2017) Substrate-analog binding and electrostatic surfaces of human manganese superoxide dismutase. J Struct Biol 199:68-75|
|Bonham-Carter, Oliver; Thapa, Ishwor; From, Steven et al. (2017) A study of bias and increasing organismal complexity from their post-translational modifications and reaction site interplays. Brief Bioinform 18:69-84|
Showing the most recent 10 out of 322 publications