Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Construction of an inducible plasmid: For regulated expression of enhanced green fluorescent protein (EGFP), lentiviral vectors with doxycycline inducible promoter system based on hybrid repressor tetR-VP16 or tetR-KRAB can be used. In absence of doxycycline, the hybrid repressor will bind specifically to tetO and suppresses the nearby promoter controlling EGFP expression. Conversely, in the presence of doxycycline, the repressor will be sequestered away from tetO, thus permitting expression of EGFP. tetR: tetracyclin repressor; VP16: HSV VP16 transactivator domain; KRAB: KRAB domain of human Kox1; tetO: tet operator sequenceOnce the constructs are ready the hESC will be transfected and checked for GFP expression ad injectedin to E13.5 mouse embryonic kidney and organ cultured for 7 days. The kidenys will then be subjected to immno-histochemical analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016475-08
Application #
7720198
Study Section
Special Emphasis Panel (ZRR1-RI-7 (02))
Project Start
2008-05-01
Project End
2009-04-30
Budget Start
2008-05-01
Budget End
2009-04-30
Support Year
8
Fiscal Year
2008
Total Cost
$32,204
Indirect Cost

  Institution

Name
University of Kansas
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160

  Publications

Paper, Janet M; Mukherjee, Thiya; Schrick, Kathrin (2018) Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants. Plant Methods 14:31
McCarson, Kenneth E; Winter, Michelle K; Abrahamson, Dale R et al. (2018) Assessing complex movement behaviors in rodent models of neurological disorders. Neurobiol Learn Mem :
Rettig, Trisha A; Ward, Claire; Bye, Bailey A et al. (2018) Characterization of the naive murine antibody repertoire using unamplified high-throughput sequencing. PLoS One 13:e0190982
Arisz, Steven A; Heo, Jae-Yun; Koevoets, Iko T et al. (2018) DIACYLGLYCEROL ACYLTRANSFERASE1 Contributes to Freezing Tolerance. Plant Physiol 177:1410-1424
Lee, Sungsu; Cheung-See-Kit, Melanie; Williams, Tyler A et al. (2018) The glycosomal alkyl-dihydroxyacetonephosphate synthase TbADS is essential for the synthesis of ether glycerophospholipids in procyclic trypanosomes. Exp Parasitol 185:71-78
Ishiguro, Susumu; Kawabata, Atsushi; Zulbaran-Rojas, Alejandro et al. (2018) Co-treatment with a C1B5 peptide of protein kinase C? and a low dose of gemcitabine strongly attenuated pancreatic cancer growth in mice through T cell activation. Biochem Biophys Res Commun 495:962-968
Haimov, Ora; Sehrawat, Urmila; Tamarkin-Ben Harush, Ana et al. (2018) Dynamic interactions of eIF4G1 with eIF4E and eIF1 underlie scanning dependent and independent translation. Mol Cell Biol :
Murakami, Ryo; Singh, Chingakham Ranjit; Morris, Jacob et al. (2018) The Interaction between the Ribosomal Stalk Proteins and Translation Initiation Factor 5B Promotes Translation Initiation. Mol Cell Biol 38:
Lee, Soon Goo; Jez, Joseph M (2017) Conformational changes in the di-domain structure of Arabidopsis phosphoethanolamine methyltransferase leads to active-site formation. J Biol Chem 292:21690-21702
Pook, Victoria G; Nair, Meera; Ryu, KookHui et al. (2017) Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots. Sci Rep 7:5714

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