This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the budding yeast Saccharomyces cerevisiae, large-scale gene deletion analysis has shown that over 80% of the ~6200 yeast genes are non-essential, implying that many genes and pathways in eukaryotic cells must be functionally redundant or buffered from phenotypic consequences following genetic perturbation. I am interested in exploring this functional redundancy, especially as it relates to transcriptional regulatory networks. A number of uncharacterized open reading frames (ORFs) have sequence homology to well known families of transcription factors. Using high-throughput genetic analysis (Synthetic Genetic Array Analysis, or SGA), DNA microarrays, and Genome-wide location analysis or Chip on chip, I would like to connect one or more of these uncharacterized putative regulatory genes with a new or existing physiologically significant pathway.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Exploratory Grants (P20)
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Special Emphasis Panel (ZRR1-RI-7 (01))
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University of Southern Mississippi
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Amato, Douglas V; Amato, Dahlia N; Blancett, Logan T et al. (2018) A bio-based pro-antimicrobial polymer network via degradable acetal linkages. Acta Biomater 67:196-205
Dutta, Shovan; Celestine, Michael J; Khanal, Supreet et al. (2018) Coordination of different ligands to copper(II) and cobalt(III) metal centers enhances Zika virus and dengue virus loads in both arthropod cells and human keratinocytes. Biochim Biophys Acta Gen Subj 1862:40-50
Budachetri, Khemraj; Kumar, Deepak; Crispell, Gary et al. (2018) The tick endosymbiont Candidatus Midichloria mitochondrii and selenoproteins are essential for the growth of Rickettsia parkeri in the Gulf Coast tick vector. Microbiome 6:141
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Budachetri, Khemraj; Williams, Jaclyn; Mukherjee, Nabanita et al. (2017) The microbiome of neotropical ticks parasitizing on passerine migratory birds. Ticks Tick Borne Dis 8:170-173
Budachetri, K; Kumar, D; Karim, S (2017) Catalase is a determinant of the colonization and transovarial transmission of Rickettsia parkeri in the Gulf Coast tick Amblyomma maculatum. Insect Mol Biol 26:414-419
Budachetri, Khemraj; Crispell, Gary; Karim, Shahid (2017) Amblyomma maculatum SECIS binding protein 2 and putative selenoprotein P are indispensable for pathogen replication and tick fecundity. Insect Biochem Mol Biol 88:37-47
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