This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Laser immunotherapy is a novel modality, which utilizes the direct tumor destruction by selective phototherapies and the indirect, long-term effect of immunological stimulation, for the treatment of metastatic tumors. The combination of photoirradiation and in situ applications of immunoadjuvants has induced tumor-specific immunity in previous animals studies. The overall objective of this project is to optimize the modality and to understand its biological mechanism. Methods: Magnetic Resonance Imaging (MRI) technique was used to monitor selective laser thermal treatment of phantom gel, chicken tissue and mouse tumors in order to test the hypothesis of temperature control in laser treatment. Two novel immunoadjuvants, glycated chitosan (GC) and imiquimod (IMQ), were intralesionally injected to mice bearing B16 melanoma and the responses of the animals were studied. To further study the effect of the combination of laser of immunoadjuvant, photodynamic therapy (PDT) and glycated chitosan were used in the treatment of EMT6 mammary tumors and Line 1 lung tumors in mice. Results: The MRI images of the target tissue and animal tumors during laser irradiation showed clear tissue structure changes and temperature distribution. Animal experiments showed that when the GC and IMQ were used together, the tumor growth was significantly slowed. In the treatment of EMT6 mammary sarcoma in mice, GC of 0.5% and 1.5% concentrations increased the cure rates of Photofrin-based PDT treatment from 38% to 63% and 75%, respectively. In the treatment of Line 1 lung adenocarcinoma in mice, a 1.67% GC solution enabled a non-curative mTHPC-based PDT to cure 37% of the tumor bearing mice. Conclusion: Following the current studies, it is expected that the control of tissue temperature during laser irradiation can be achieved, making the optimization of thermal treatment possible. Future study will also demonstrate the enhancement of immune responses in tumor treatment using the combination of laser and immunoadjuvants. Ultimately, this method could be applied clinically for the patients with metastatic tumors.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016478-06
Application #
7381639
Study Section
Special Emphasis Panel (ZRR1-RI-7 (02))
Project Start
2006-05-01
Project End
2007-04-30
Budget Start
2006-05-01
Budget End
2007-04-30
Support Year
6
Fiscal Year
2006
Total Cost
$111,355
Indirect Cost
Name
University of Oklahoma Health Sciences Center
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
878648294
City
Oklahoma City
State
OK
Country
United States
Zip Code
73117
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2018) Modeling Transcriptional Rewiring in Neutrophils Through the Course of Treated Juvenile Idiopathic Arthritis. Sci Rep 8:7805
Wetherill, Marianna S; Williams, Mary B; Gray, Karen A (2017) SNAP-Based Incentive Programs at Farmers' Markets: Adaptation Considerations for Temporary Assistance for Needy Families (TANF) Recipients. J Nutr Educ Behav 49:743-751.e1
Hannafon, Bethany N; Trigoso, Yvonne D; Calloway, Cameron L et al. (2016) Plasma exosome microRNAs are indicative of breast cancer. Breast Cancer Res 18:90
Wilson, Kevin R; Cannon-Smith, Desiray J; Burke, Benjamin P et al. (2016) Synthesis and structural studies of two pyridine-armed reinforced cyclen chelators and their transition metal complexes. Polyhedron 114:118-127
Trigoso, Yvonne D; Evans, Russell C; Karsten, William E et al. (2016) Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase. PLoS One 11:e0146525
Khandaker, Morshed; Riahinezhad, Shahram; Sultana, Fariha et al. (2016) Peen treatment on a titanium implant: effect of roughness, osteoblast cell functions, and bonding with bone cement. Int J Nanomedicine 11:585-94
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2016) Complexity and Specificity of the Neutrophil Transcriptomes in Juvenile Idiopathic Arthritis. Sci Rep 6:27453
Matz, Dallas L; Jones, Donald G; Roewe, Kimberly D et al. (2015) Synthesis, structural studies, kinetic stability, and oxidation catalysis of the late first row transition metal complexes of 4,10-dimethyl-1,4,7,10-tetraazabicyclo[6.5.2]pentadecane. Dalton Trans 44:12210-24
Zhang, Shuyu; Xue, Jing; Zheng, Jie et al. (2015) The superoxide dismutase 1 3'UTR maintains high expression of the SOD1 gene in cancer cells: The involvement of the RNA-binding protein AUF-1. Free Radic Biol Med 85:33-44
Wang, Shuai; Hannafon, Bethany N; Lind, Stuart E et al. (2015) Zinc Protoporphyrin Suppresses ?-Catenin Protein Expression in Human Cancer Cells: The Potential Involvement of Lysosome-Mediated Degradation. PLoS One 10:e0127413

Showing the most recent 10 out of 165 publications