This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Improper maintenance of ploidy (chromosome number) is a hallmark of many cancer cell types, and is thought to contribute to the malignancy of cancer cell populations. In a number of tumors tetraploidy (exactly twice the normal chromosome number) precedes a more degenerate state, in which individual chromosomes are lost or gained at random. With this project we begin an investigation of a particular chromosomal arrangement that forms in some tetraploid cells, called a diplochromosome.Diplochromosomes are most commonly seen in tumor cells, in cells that have been treated with certain kinds of poisons, and in cells from individuals with defects in sister chromatid cohesion. The conditions that lead to diplochromosome formation are not understood. The segregation of diplochromosomes when cells divide has not been investigated in detail, but studies in some organisms suggest that it is highly error-prone. Thus diplochromosomes might contribute to development of the CIN phenotype, and explain the connection between tetraploidy and malignancy. The significance of the presence of diplochromosomes, and the factors that contribute to their formation, have not been investigated. This project includes experiments that will allow us to begin to address both issues. We will also determine the segregation behavior of diplochromosomes at cell division. With these experiments we will increase our understanding of the etiology of certain human developmental disorders, in which decreased sister chromatid cohesion leads both to formation of diplochromosome-containing cells and increased incidence of cancer.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016478-08
Application #
7725104
Study Section
Special Emphasis Panel (ZRR1-RI-7 (02))
Project Start
2008-05-01
Project End
2009-04-30
Budget Start
2008-05-01
Budget End
2009-04-30
Support Year
8
Fiscal Year
2008
Total Cost
$101,667
Indirect Cost
Name
University of Oklahoma Health Sciences Center
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
878648294
City
Oklahoma City
State
OK
Country
United States
Zip Code
73117
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2018) Modeling Transcriptional Rewiring in Neutrophils Through the Course of Treated Juvenile Idiopathic Arthritis. Sci Rep 8:7805
Wetherill, Marianna S; Williams, Mary B; Gray, Karen A (2017) SNAP-Based Incentive Programs at Farmers' Markets: Adaptation Considerations for Temporary Assistance for Needy Families (TANF) Recipients. J Nutr Educ Behav 49:743-751.e1
Hannafon, Bethany N; Trigoso, Yvonne D; Calloway, Cameron L et al. (2016) Plasma exosome microRNAs are indicative of breast cancer. Breast Cancer Res 18:90
Wilson, Kevin R; Cannon-Smith, Desiray J; Burke, Benjamin P et al. (2016) Synthesis and structural studies of two pyridine-armed reinforced cyclen chelators and their transition metal complexes. Polyhedron 114:118-127
Trigoso, Yvonne D; Evans, Russell C; Karsten, William E et al. (2016) Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase. PLoS One 11:e0146525
Khandaker, Morshed; Riahinezhad, Shahram; Sultana, Fariha et al. (2016) Peen treatment on a titanium implant: effect of roughness, osteoblast cell functions, and bonding with bone cement. Int J Nanomedicine 11:585-94
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2016) Complexity and Specificity of the Neutrophil Transcriptomes in Juvenile Idiopathic Arthritis. Sci Rep 6:27453
Matz, Dallas L; Jones, Donald G; Roewe, Kimberly D et al. (2015) Synthesis, structural studies, kinetic stability, and oxidation catalysis of the late first row transition metal complexes of 4,10-dimethyl-1,4,7,10-tetraazabicyclo[6.5.2]pentadecane. Dalton Trans 44:12210-24
Zhang, Shuyu; Xue, Jing; Zheng, Jie et al. (2015) The superoxide dismutase 1 3'UTR maintains high expression of the SOD1 gene in cancer cells: The involvement of the RNA-binding protein AUF-1. Free Radic Biol Med 85:33-44
Wang, Shuai; Hannafon, Bethany N; Lind, Stuart E et al. (2015) Zinc Protoporphyrin Suppresses ?-Catenin Protein Expression in Human Cancer Cells: The Potential Involvement of Lysosome-Mediated Degradation. PLoS One 10:e0127413

Showing the most recent 10 out of 165 publications