This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The long-term objective of this proposed project is to determine functions of TGF-beta in cancer survellience and intervention and to improve the effectiveness of a novel laser immunotherapy by reducing TGF-beta levels in teh tumor cells. Laser immunotherapy was developed by the PIs, using a near-infrared laser, indocyanine green (ICG, a light-absorbing dye), and blycated chitosan (GC, a proprietary immunoadjuvant) for treatment of metastatic tumors. Our previous studies using laser immunotherapy have shown extremely promising outcomes in the treatment of late-stage, metastatic tumors, both in animal models and in human trials for melanoma and breast tumors. TGF-beta has been shown to be a crucial cytokine in cancer surveillance and intervention. It is hypothesized that reducing TGF-beta levels in tumor microenvironment could significantly enhance the outcomes of laser immunotherapy in treating metastatic tumors through the enhanced anti-tumor immune responses. In this study, we plan to achieve the following specific aims: (1) to reduce TGF-beta levels in DMBA-4 tumor cells for in vitro ain in vivo studies;(2) to determine the effects of TGF-beta reduction on laser immunotherapy treatment of metastatic tumors in rats;(3) to investigate the effects of TGF-beta reducatino on immune cells in the tumor microenvironment. This one-year collaborative grant will allow an initial investigation of the functions of TGF-beta and the mechanism of laser immunotherapy, paving he way for a large-scale, in-depth future study for TGF-beta associated tumor treatment modalities.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016478-11
Application #
8359650
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2011-04-01
Project End
2012-03-31
Budget Start
2011-04-01
Budget End
2012-03-31
Support Year
11
Fiscal Year
2011
Total Cost
$68,524
Indirect Cost
Name
University of Oklahoma Health Sciences Center
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
878648294
City
Oklahoma City
State
OK
Country
United States
Zip Code
73117
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2018) Modeling Transcriptional Rewiring in Neutrophils Through the Course of Treated Juvenile Idiopathic Arthritis. Sci Rep 8:7805
Wetherill, Marianna S; Williams, Mary B; Gray, Karen A (2017) SNAP-Based Incentive Programs at Farmers' Markets: Adaptation Considerations for Temporary Assistance for Needy Families (TANF) Recipients. J Nutr Educ Behav 49:743-751.e1
Hannafon, Bethany N; Trigoso, Yvonne D; Calloway, Cameron L et al. (2016) Plasma exosome microRNAs are indicative of breast cancer. Breast Cancer Res 18:90
Wilson, Kevin R; Cannon-Smith, Desiray J; Burke, Benjamin P et al. (2016) Synthesis and structural studies of two pyridine-armed reinforced cyclen chelators and their transition metal complexes. Polyhedron 114:118-127
Trigoso, Yvonne D; Evans, Russell C; Karsten, William E et al. (2016) Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase. PLoS One 11:e0146525
Khandaker, Morshed; Riahinezhad, Shahram; Sultana, Fariha et al. (2016) Peen treatment on a titanium implant: effect of roughness, osteoblast cell functions, and bonding with bone cement. Int J Nanomedicine 11:585-94
Hu, Zihua; Jiang, Kaiyu; Frank, Mark Barton et al. (2016) Complexity and Specificity of the Neutrophil Transcriptomes in Juvenile Idiopathic Arthritis. Sci Rep 6:27453
Matz, Dallas L; Jones, Donald G; Roewe, Kimberly D et al. (2015) Synthesis, structural studies, kinetic stability, and oxidation catalysis of the late first row transition metal complexes of 4,10-dimethyl-1,4,7,10-tetraazabicyclo[6.5.2]pentadecane. Dalton Trans 44:12210-24
Zhang, Shuyu; Xue, Jing; Zheng, Jie et al. (2015) The superoxide dismutase 1 3'UTR maintains high expression of the SOD1 gene in cancer cells: The involvement of the RNA-binding protein AUF-1. Free Radic Biol Med 85:33-44
Wang, Shuai; Hannafon, Bethany N; Lind, Stuart E et al. (2015) Zinc Protoporphyrin Suppresses ?-Catenin Protein Expression in Human Cancer Cells: The Potential Involvement of Lysosome-Mediated Degradation. PLoS One 10:e0127413

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