This study will address the central hypothesis that the synaptic physiology (MBs) of the Mushroom Bodies underlies the temporal coding that is essential for chemosensory learning of Drosophila. The MBs have been shown to be essential for associative olfactory learning. This study will utilize a living, whole-fly preparation that allows direct pharmacological manipulation of the MBs. [Ca2+]i and membrane potential of the entire MB neuronal population will be monitored simultaneously by fluorescent imaging via a window cut in the fly's head capsule. Potentiometric dyes will be used for membrane potential imaging while new, endogenous sensors (cameleon and camgaroo mutants) will be used for Ca2+ imaging. The pharmacology relevant to the processing of chemosensory information will be investigated. The effects of acetylcholine, dopamine, octopamine, GABA, serotonin, and a putative neuropeptide gene product from the memory mutant amnesiac will be tested. The novel neurotransmitters nitric oxide and carbon dioxide will also be tested. Any secondary messenger systems thought to be involved will also be investigated. This will add to our knowledge of how neuronal systems process and encode sensory information both for perception and memory formation through learning. The broader impacts of this study are many. These imaging studies are easier to implement than electrophysiological techniques so that undergraduate seniors and graduate students can be trained to use this model for student projects that will contribute to this research. Eastern New Mexico University is a Hispanic-serving institution, as designated by the Department of Education, and it is expected that minority students will be trained and contribute to this work. Imaging, especially of endogenous sensors, is a new and exciting research tool that has yielded much useful information concerning the function of neuronal populations. Any research that contributes to our understanding of learning and memory will have an impact on the health of society.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
2P20RR016480-04
Application #
6972147
Study Section
Special Emphasis Panel (ZRR1-RI-7 (02))
Project Start
2004-07-05
Project End
2005-06-30
Budget Start
2004-07-05
Budget End
2005-06-30
Support Year
4
Fiscal Year
2004
Total Cost
$137,695
Indirect Cost
Name
New Mexico State University Las Cruces
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
173851965
City
Las Cruces
State
NM
Country
United States
Zip Code
88003
Licon-Munoz, Yamhilette; Fordyce, Colleen A; Hayek, Summer Raines et al. (2018) V-ATPase-dependent repression of androgen receptor in prostate cancer cells. Oncotarget 9:28921-28934
Duplessis, Christopher; Gregory, Michael; Frey, Kenneth et al. (2018) Evaluating the discriminating capacity of cell death (apoptotic) biomarkers in sepsis. J Intensive Care 6:72
Johnston, Robert K; Harper, Jason C; Tartis, Michaelann S (2017) Control over Silica Particle Growth and Particle-Biomolecule Interactions Facilitates Silica Encapsulation of Mammalian Cells with Thickness Control. ACS Biomater Sci Eng 3:2098-2109
Rossi, Shannan L; Tesh, Robert B; Azar, Sasha R et al. (2016) Characterization of a Novel Murine Model to Study Zika Virus. Am J Trop Med Hyg 94:1362-9
Shuster, Michele; Claussen, Kira; Locke, Melly et al. (2016) BIOINFORMATICS IN THE K-8 CLASSROOM: DESIGNING INNOVATIVE ACTIVITIES FOR TEACHER IMPLEMENTATION. Int J Des Learn 7:60-70
Tsalik, Ephraim L; Henao, Ricardo; Nichols, Marshall et al. (2016) Host gene expression classifiers diagnose acute respiratory illness etiology. Sci Transl Med 8:322ra11
Colip, Leslie; Burge, Mark R; Sandy, Phillip et al. (2016) Exercise Intervention Improves the Metabolic Profile and Body Composition of Southwestern American Indian Adolescents. J Diabetes Obes 3:
Reiss, Rebecca A; Guerra, Peter; Makhnin, Oleg (2016) Metagenome phylogenetic profiling of microbial community evolution in a tetrachloroethene-contaminated aquifer responding to enhanced reductive dechlorination protocols. Stand Genomic Sci 11:88
Camacho, Jenny E; Shah, Vallabh O; Schrader, Ronald et al. (2016) PERFORMANCE OF A1C VERSUS OGTT FOR THE DIAGNOSIS OF PREDIABETES IN A COMMUNITY-BASED SCREENING. Endocr Pract 22:1288-1295
Andrade, C C; Young, K I; Johnson, W L et al. (2016) Rise and fall of vector infectivity during sequential strain displacements by mosquito-borne dengue virus. J Evol Biol 29:2205-2218

Showing the most recent 10 out of 296 publications