This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Technology Core: As previously described, all of the major resources previously obtained are fully operational and provide considerable support for our junior faculty investigators, postdoctoral fellows, and graduate students. The renovated space houses the Imaging, Immunohistochemistry and Molecular Biology resource units. Dr. Kobori, junior faculty investigator of Project # 1, is also the Director of the Molecular Core. The analytical facility performs RIA and ELIZA analysis of plasma, tissue and urine samples of several agents, including renin, angiotensin I and angiotensin II. The gel documentation system allows rapid quantification of DNA, RNA, and protein samples. The other component under the direction of Dr. Hammond continues to provide for both gene array and flow cytometry studies. During the previous period we added deletion series array analysis to the genetic analysis expertise and we worked on better methods for confirmation of gene array using real time PCR and microfluidic technologies.
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