This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.To allow Dr. Faqian Li to focus more on his project, Dr. Steven Armstrong was recently appointed Director of this core late in year 3. Dr. Armstrong has extensive experience in confocal microscopy and his office is located directly across the hall from the confocal room. Dr. Armstrong also spearheaded an NIH equipment grant for a new confocal microscope, which has been resubmitted in year 4. Proposed projects 1, 2, and 3 require confocal microscopy. The confocal microscope was updated with new software. The old computer for the confocal system is now a working station to process images acquired by the confocal system. We have extensively investigated the translocation of G-protein coupled receptor kinases and focal adhesion kinase during cardiac hypertrophy and failure. The cellular distribution of signaling molecules in cultured cardiac myocytes transfected with adenoviral constructs has been analyzed. We established an H-E staining station. We also provide frozen sectioning for PIs. The imaging core also provides technical training and expertise for investigators doing their own confocal microscopy.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
2P20RR017662-06A1
Application #
7720650
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Project Start
2008-07-01
Project End
2009-06-30
Budget Start
2008-07-01
Budget End
2009-06-30
Support Year
6
Fiscal Year
2008
Total Cost
$104,258
Indirect Cost
Name
University of South Dakota
Department
Other Basic Sciences
Type
Schools of Medicine
DUNS #
929930808
City
Vermillion
State
SD
Country
United States
Zip Code
57069
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