Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. EPR (also referred to as Electron Spin Resonance) spectroscopy is based on absorption of microwave energy by free radicals or paramagnetic species in the presence of an external electromagnetic field resulting in the transition of electron spin states. Dr. Zimmerman's laboratory in the Department of Cellular and Integrative Physiology at UNMC houses a Bruker E-Scan Table Top EPR spectrometer (see figure). In combination with EPR-detectable, radical-sensitive cyclic hydroxylamines, often referred to as """"""""spin probes"""""""", the E-Scan can be and has been used to measure levels of free radicals (e.g. superoxide, hydroxyl radical) in various biological samples including cultured cells, culture media, blood, and isolated tissue.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR017675-10
Application #
8360536
Study Section
Special Emphasis Panel (ZRR1-RI-5 (01))
Project Start
2011-08-01
Project End
2012-08-31
Budget Start
2011-08-01
Budget End
2013-07-31
Support Year
10
Fiscal Year
2011
Total Cost
$13,468
Indirect Cost

  Institution

Name
University of Nebraska Lincoln
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
555456995
City
Lincoln
State
NE
Country
United States
Zip Code
68588

  Publications

Garza-Lombó, Carla; Schroder, Annika; Reyes-Reyes, Elsa M et al. (2018) mTOR/AMPK signaling in the brain: Cell metabolism, proteostasis and survival. Curr Opin Toxicol 8:102-110
Markley, John L; Brüschweiler, Rafael; Edison, Arthur S et al. (2017) The future of NMR-based metabolomics. Curr Opin Biotechnol 43:34-40
Duszenko, Nikolas; Buan, Nicole R (2017) Physiological Evidence for Isopotential Tunneling in the Electron Transport Chain of Methane-Producing Archaea. Appl Environ Microbiol 83:
Anandhan, Annadurai; Jacome, Maria S; Lei, Shulei et al. (2017) Metabolic Dysfunction in Parkinson's Disease: Bioenergetics, Redox Homeostasis and Central Carbon Metabolism. Brain Res Bull 133:12-30
Marshall, Darrell D; Powers, Robert (2017) Beyond the paradigm: Combining mass spectrometry and nuclear magnetic resonance for metabolomics. Prog Nucl Magn Reson Spectrosc 100:1-16
Anandhan, Annadurai; Lei, Shulei; Levytskyy, Roman et al. (2017) Glucose Metabolism and AMPK Signaling Regulate Dopaminergic Cell Death Induced by Gene (?-Synuclein)-Environment (Paraquat) Interactions. Mol Neurobiol 54:3825-3842
Rose, Jordan; Brian, Christian; Woods, Jade et al. (2017) Mitochondrial dysfunction in glial cells: Implications for neuronal homeostasis and survival. Toxicology 391:109-115
Boone, Cory H T; Grove, Ryan A; Adamcova, Dana et al. (2017) Oxidative stress, metabolomics profiling, and mechanism of local anesthetic induced cell death in yeast. Redox Biol 12:139-149
Gebregiworgis, Teklab; Nielsen, Helle H; Massilamany, Chandirasegaran et al. (2016) A Urinary Metabolic Signature for Multiple Sclerosis and Neuromyelitis Optica. J Proteome Res 15:659-66
Navarro-Yepes, Juliana; Anandhan, Annadurai; Bradley, Erin et al. (2016) Inhibition of Protein Ubiquitination by Paraquat and 1-Methyl-4-Phenylpyridinium Impairs Ubiquitin-Dependent Protein Degradation Pathways. Mol Neurobiol 53:5229-51

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