This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core C, the Molecular Biology Support facility, provides the interface, expertise, training and mentoring in gene array analysis necessary to advance COBRE projects. Gene array technology provides a means to screen individual cell types for gene expression patterns and changes in a highly efficient manner. Gene microarray processing and bioinformatics services are available through the auspices of another NIH grant to Kansas, thereby allowing us to focus the support from this Core on support for sample preparation (analyses of quality and quantity), funding of off-site gene microarray processing and post-gene microarray validation (qRT-PCR). The Core provides gene array evaluations by outsourcing investigator RNA samples for gene chip preparation and hybridization to another facility, along with the Bioinformatics support to process the data. The Core provides support through: 1) two, real-time polymerase chain reaction (PCR) thermocyclers for quantitative assessment of gene transcripts, 2) an Agilent BioAnalyzer for determining the quality of RNA samples obtained for gene array analysis, 3) a Nanodrop spectrophotometer and spectrofluorometer for determining the quantity of RNA samples, and 4) a DNA sequencer for verifying PCR product identity. The real-time PCR is needed to verify gene array results or analyze specific gene expression changes in more detail than is possible with the gene array.
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